ArticleThe Journal of biological chemistry2003
Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells.
Article in The Journal of biological chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 85 citations in OpenAlex.
- FLOT1 promotes gastric cancer progression and metastasis through BCAR1/ERK signaling.International journal of biological sciences · 2023Article
- Engineered kinases as a tool for phosphorylation of selected targets in vivo.The Journal of cell biology · 2022Article
- Independent effects of Src kinase and podoplanin on anchorage independent cell growth and migration.Molecular carcinogenesis · 2022Article
- Heterocellular N-cadherin junctions enable nontransformed cells to inhibit the growth of adjacent transformed cells.Cell communication and signaling : CCS · 2022Article
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- New Structural Perspectives in G Protein-Coupled Receptor-Mediated Src Family Kinase Activation.International journal of molecular sciences · 2021Review
- Src activation by Chk1 promotes actin patch formation and prevents chromatin bridge breakage in cytokinesis.The Journal of cell biology · 2018Article
- Article
- Mind the gap: connexins and cell-cell communication in the diabetic kidney.Diabetologia · 2015Review
- Preferred SH3 domain partners of ADAM metalloproteases include shared and ADAM-specific SH3 interactions.PloS one · 2015Article
- Identification of direct tyrosine kinase substrates based on protein kinase assay-linked phosphoproteomics.Molecular & cellular proteomics : MCP · 2013Article
- Serines in the intracellular tail of podoplanin (PDPN) regulate cell motility.The Journal of biological chemistry · 2013Article
- Identification and functional characterization of p130Cas as a substrate of protein tyrosine phosphatase nonreceptor 14.Oncogene · 2013Article
- Article
- Article
- Src, p130Cas, and Mechanotransduction in Cancer Cells.Genes & cancer · 2012Article
- SRC points the way to biomarkers and chemotherapeutic targets.Genes & cancer · 2012Article
- Podoplanin expression during dysplasia-carcinoma sequence in the oral cavity.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2012Article
- The role of Src kinase in macrophage-mediated inflammatory responses.Mediators of inflammation · 2012Review
- Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Cas is a member of the focal adhesion complex. Phosphorylation of Cas by Src is an important event leading to cell transformation. Using mass spectrometry, we have mapped 11 sites in Cas that are phosphorylated by Src. These sites are all located between residues 132 and 414 of Cas, in a region that is required for binding to a number of other proteins including Crk. We tested synthetic peptides modeled on Cas phosphorylation sites, and found that the sequence containing tyrosine 253 was phosphorylated by Src most efficiently. Using cells derived from Cas-deficient mice, we confirmed that Cas greatly enhanced the ability of Src to transform cells. Phosphorylation of Cas on tyrosine 253 was not required for Src to increase growth rate, suppress contact inhibition, or suppress anchorage dependence. Yet, in contrast to these growth characteristics, phosphorylation of Cas on tyrosine 253 was required for Src to promote cell migration. Thus, a single phosphorylation site on this focal adhesion adaptor protein can effectively separate cell migration from other transformed growth characteristics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.