ArticleWorld journal of gastroenterology2003
Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells.
Article in World journal of gastroenterology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Bibliometric analysis of nanomaterials in hepatocellular carcinoma treatment: research trends, knowledge structures, and emerging insights (2000-2024).Discover oncology · 2025Article
- Hydroxyapatite Biobased Materials for Treatment and Diagnosis of Cancer.International journal of molecular sciences · 2022Review
- Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior.International journal of molecular sciences · 2022Article
- Anti-melanoma effect and action mechanism of a novel chitosan-based composite hydrogel containing hydroxyapatite nanoparticles.Regenerative biomaterials · 2022Article
- In Vitro Biocompatibility Assessment of Nano-Hydroxyapatite.Nanomaterials (Basel, Switzerland) · 2021Article
- Evaluating the Radiosensitization Effect of Hydroxyapatite Nanoparticles on Human Breast Adenocarcinoma Cell Line and Fibroblast.Iranian journal of medical sciences · 2020Article
- Preparation, characterization, and anticancer efficacy of novel cobalt oxide nanoparticles conjugated with thiosemicarbazide.3 Biotech · 2020Article
- Biodegradable calcium phosphate nanoparticles for cancer therapy.Advances in colloid and interface science · 2020Review
- 3D Microfluidic Bone Tumor Microenvironment Comprised of Hydroxyapatite/Fibrin Composite.Frontiers in bioengineering and biotechnology · 2019Article
- Crystallinity of hydroxyapatite drives myofibroblastic activation and calcification in aortic valves.Acta biomaterialia · 2018Article
- Fabrication and Use of Poly(d,l-lactide-co-glycolide)-Based Formulations Designed for Modified Release of 5-Fluorouracil.Journal of pharmaceutical sciences · 2018Review
- The inhibition of lamellar hydroxyapatite and lamellar magnetic hydroxyapatite on the migration and adhesion of breast cancer cells.Journal of materials science. Materials in medicine · 2014Article
- Anti-hepatoma effect of safrole from Cinnamomum longepaniculatum leaf essential oil in vitro.International journal of clinical and experimental pathology · 2014Article
- Amorphous calcium phosphate nanoparticles could function as a novel cancer therapeutic agent by employing a suitable targeted drug delivery platform.Nanoscale research letters · 2013Article
- Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo.International journal of nanomedicine · 2012Article
- Characteristics of functionalized nano-hydroxyapatite and internalization by human epithelial cell.Nanoscale research letters · 2011Article
- Induced apoptotic action of recombinant trichosanthin in human stomach adenocarcinoma MCG803 cells.Molecular biology reports · 2009Article
- Influence of spray-dried hydroxyapatite-5-fluorouracil granules on cell lines derived from tissues of mesenchymal origin.Molecules (Basel, Switzerland) · 2008Article
- Effect of hydroxyapatite nanoparticles on the growth and p53/c-Myc protein expression of implanted hepatic VX2 tumor in rabbits by intravenous injection.World journal of gastroenterology · 2007Article
- Effect of extracts of trichosanthes root tubers on HepA-H cells and HeLa cells.World journal of gastroenterology · 2004Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo study the effect of hydroxyapatite (HAP) nanoparticles on human hepatoma cell line BEL-7402 in vitro.
methodsThe human hepatoma cell line BEL-7402 was cultured and treated with HAP nanoparticles at various concentrations. Growth suppression was detected with MTT colorimetric assay, cell apoptotic alterations were evaluated by cytochemical staining (Hoechst 33258), transmission electron microscopy (TEM), and flow cytometry (FCM).
resultsHAP nanoparticles inhibited the growth of hepatoma cells in a dose-dependent manner, with IC(50) values of 29.30 mg/L. Treated with 50-200 mg/L HAP nanoparticles for 48 h, BEL-7402 cells apoptosis with nuclear chromatin condensation and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed under cytochemical staining and transmission electron microscopy. FCM analysis showed hypodiploid peaks on histogram, the apoptotic rates at the concentrations of 50, 75, 100, 150 and 200 mg/L of HAP nanoparticles were 20.35+/-2.23 %, 25.35+/-1.92 %, 29.34+/-4.61 %, 44.92+/-3.78 % and 53.64+/-3.49 %, respectively, which were all significantly higher than that of control group 2.23+/-0.14 %. There was a significant correlation between HAP nanoparticle concentration and apoptotic rate (r=0.994, P<0.01).
conclusionHAP nanoparticles not only inhibit proliferation but also induce apoptosis of human hepatoma cell line BEL-7402 in vitro.
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