Evidence map›Paper›PMID 12970500›Full record

ArticlePlant physiology2003

The gene expression and enzyme activity of plant 3-deoxy-D-manno-2-octulosonic acid-8-phosphate synthase are preferentially associated with cell division in a cell cycle-dependent manner.

Frédéric Delmas, Johann Petit, Jérôme Joubès, Martial Séveno, Thomas Paccalet, Michel Hernould, Patrice Lerouge, Armand Mouras, Christian Chevalier

Open access · bronzeAbstract read
In one paragraph

Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Article
  3. Breeding science · 2021
    Article
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  5. Article
  6. Article
  7. Article
  8. Article
  9. Glycoprotein sialylation in plants?Nature biotechnology · 2004
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Frédéric DelmasUnité Mixte de Recherche 619 de Physiologie et Biotechnologie Végétales, Institut de Biologie Végétale Moléculaire, Centre de Recherche Institut National de la Recherche Agronomique-Bordeaux, boite postale 81, 33883 Villenave d'Ornon cedex, France.
Johann Petit
Jérôme Joubès
Martial Séveno
Thomas Paccalet
Michel Hernould
Patrice Lerouge
Armand Mouras
Christian Chevalier
Unité de Recherche Œnologie · FRCentre National de la Recherche Scientifique · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

3-deoxy-D-manno-2-octulosonic acid-8-phosphate (Kdo-8-P) synthase catalyzes the condensation of phosphoenolpyruvate with D-arabinose-5-phosphate to yield Kdo-8-P. Kdo-8-P is the phosphorylated precursor of Kdo, a rare sugar only found in the rhamnogalacturonan II pectic fraction of the primary cell walls of higher plants and of cell wall polysaccharides of some green algae. A cDNA named LekdsA (accession no. AJ294902) encoding tomato (Lycopersicon esculentum) Kdo-8-P synthase has been isolated. The recombinant protein rescued a kdsA thermosensitive mutant of Salmonella typhimurium impaired in the synthesis of a functional Kdo-8-P synthase. Using site-directed mutagenesis of LekdsA cDNA, the tomato Kdo-8-P synthase was shown to possess the same essential amino acids that form the active sites in the bacterial enzymes. The tomato kdsA gene expression and the relevant Kdo-8-P synthase activity were preferentially associated to dividing cells, in the course of the early development of tomato fruit and in meristematic tissues. Furthermore, the transcription of the kdsA gene was found to oscillate during the cell cycle in tobacco (Nicotiana tabacum) Bright-Yellow 2 synchronized cells with a maximum during mitosis.

Indexed as

Aldehyde-LyasesAmino Acid SequenceCell CycleCell DivisionCloning, MolecularDNA, ComplementaryFruitGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicGene Expression Regulation, PlantHypocotylMolecular Sequence DataMutagenesis, Site-DirectedMutationNicotianaPlant Proteins2-dehydro-3-deoxyphosphooctonate aldolaseAldehyde-LyasesDNA, ComplementaryPlant ProteinsRNA, Messenger

Identifiers

PMID12970500
PMCPMC196611
OpenAlexW2094204514

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.