ArticlePlant physiology2003
Does lowering glutamine synthetase activity in nodules modify nitrogen metabolism and growth of Lotus japonicus?
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 70 citations in OpenAlex.
- Identification and characterization of the glutamine synthetase gene family in oat (Frontiers in plant science · 2025Article
- Systematic characterization of Gossypium GLN family genes reveals a potential function of GhGLN1.1a regulates nitrogen use efficiency in cotton.BMC plant biology · 2024Article
- Bacteroid Development, Transcriptome, and Symbiotic Nitrogen-Fixing Comparison of Bradyrhizobium arachidis in Nodules of Peanut (Arachis hypogaea) and Medicinal Legume Sophora flavescens.Microbiology spectrum · 2023Article
- Identification of the glutamine synthetase (GS) gene family in four wheat species and functional analysis of Ta4D.GSe in Arabidopsis thaliana.Plant molecular biology · 2022Article
- Review
- Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency.Biological research · 2020Review
- Comprehensive Identification and Expression Profiling of Circular RNAs During Nodule Development inFrontiers in plant science · 2020Article
- Impact of phenanthrene on primary metabolite profiling in root exudates and maize mucilage.Environmental science and pollution research international · 2020Article
- Article
- A Natural Light/Dark Cycle Regulation of Carbon-Nitrogen Metabolism and Gene Expression in Rice Shoots.Frontiers in plant science · 2016Article
- Article
- Herbicide phosphinothricin causes direct stimulation hormesis.Dose-response : a publication of International Hormesis Society · 2012Article
- Glutamine synthetase in legumes: recent advances in enzyme structure and functional genomics.International journal of molecular sciences · 2012Review
- A quantitative genetic study for elucidating the contribution of glutamine synthetase, glutamate dehydrogenase and other nitrogen-related physiological traits to the agronomic performance of common wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Crystallization and preliminary X-ray diffraction studies of maleylacetate reductase from Rhizobium sp. strain MTP-10005.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Article
- How rhizobial symbionts invade plants: the Sinorhizobium-Medicago model.Nature reviews. Microbiology · 2007Review
- Article
- Two cytosolic glutamine synthetase isoforms of maize are specifically involved in the control of grain production.The Plant cell · 2006Article
- Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine.The Plant cell · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding cytosolic glutamine synthetase (GS) from Lotus japonicus was fused in the antisense orientation relative to the nodule-specific LBC3 promoter of soybean (Glycine max) and introduced into L. japonicus via transformation with Agrobacterium tumefaciens. Among the 12 independent transformed lines into which the construct was introduced, some of them showed diminished levels of GS1 mRNA and lower levels of GS activity. Three of these lines were selected and their T(1) progeny was further analyzed both for plant biomass production and carbon and nitrogen (N) metabolites content under symbiotic N-fixing conditions. Analysis of these plants revealed an increase in fresh weight in nodules, roots and shoots. The reduction in GS activity was found to correlate with an increase in amino acid content of the nodules, which was primarily due to an increase in asparagine content. Thus, this study supports the hypothesis that when GS becomes limiting, other enzymes (e.g. asparagine synthetase) that have the capacity to assimilate ammonium may be important in controlling the flux of reduced N in temperate legumes such as L. japonicus. Whether these alternative metabolic pathways are important in the control of plant biomass production still remains to be fully elucidated.
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Registered trials
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