ArticleJournal of virology2003
Enhanced virulence mediated by the murine coronavirus, mouse hepatitis virus strain JHM, is associated with a glycine at residue 310 of the spike glycoprotein.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 79 citations in OpenAlex.
- Adaptive variations in SARS-CoV-2 spike proteins: effects on distinct virus-cell entry stages.mBio · 2023Article
- Antiviral performance of graphene-based materials with emphasis on COVID-19: A review.Medicine in drug discovery · 2021Review
- Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control Elements in the Amino-Terminal Domains.mBio · 2021Article
- Potential neuroinvasive and neurotrophic properties of SARS-CoV-2 in pediatric patients: comparison of SARS-CoV-2 with non-segmented RNA viruses.Journal of neurovirology · 2020Article
- Immuno-epidemiology and pathophysiology of coronavirus disease 2019 (COVID-19).Journal of molecular medicine (Berlin, Germany) · 2020Review
- Distinct viral clades of SARS-CoV-2: Implications for modeling of viral spread.Journal of medical virology · 2020Article
- Review
- Identification of H209 as Essential for pH 8-Triggered Receptor-Independent Syncytium Formation by S Protein of Mouse Hepatitis Virus A59.Journal of virology · 2018Article
- Proteolytic processing of Middle East respiratory syndrome coronavirus spikes expands virus tropism.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structure, Function, and Evolution of Coronavirus Spike Proteins.Annual review of virology · 2016Review
- Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice.Archives of virology · 2015Article
- The nsp1, nsp13, and M proteins contribute to the hepatotropism of murine coronavirus JHM.WU.Journal of virology · 2015Article
- The nsp3 macrodomain promotes virulence in mice with coronavirus-induced encephalitis.Journal of virology · 2015Article
- Role of the spike glycoprotein of human Middle East respiratory syndrome coronavirus (MERS-CoV) in virus entry and syncytia formation.PloS one · 2013Article
- Review
- Virally expressed interleukin-10 ameliorates acute encephalomyelitis and chronic demyelination in coronavirus-infected mice.Journal of virology · 2011Article
- Spongiform degeneration induced by neuropathogenic murine coronavirus infection.Pathology international · 2011Article
- Pathogenesis of neurotropic murine coronavirus is multifactorial.Trends in pharmacological sciences · 2011Article
- Coronavirus pathogenesis.Advances in virus research · 2011Review
- Murine coronavirus receptors are differentially expressed in the central nervous system and play virus strain-dependent roles in neuronal spread.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The coronavirus, mouse hepatitis virus strain JHM, causes acute and chronic neurological diseases in rodents. Here we demonstrate that two closely related virus variants, both of which cause acute encephalitis in susceptible strains of mice, cause markedly different diseases if mice are protected with a suboptimal amount of an anti-JHM neutralizing antibody. One strain, JHM.SD, caused acute encephalitis, while infection with JHM.IA resulted in no acute disease. Using recombinant virus technology, we found that the differences between the two viruses mapped to the spike (S) glycoprotein and that the two S proteins differed at four amino acids. By engineering viruses that differed by only one amino acid, we identified a serine-to-glycine change at position 310 of the S protein (S310G) that recapitulated the more neurovirulent phenotype. The increased neurovirulence mediated by the virus encoding glycine at position S310 was not associated with a different tropism within the central nervous system (CNS) but was associated with increased lateral spread in the CNS, leading to significantly higher brain viral titers. In vitro studies revealed that S310G was associated with decreased S1-S2 stability and with enhanced ability to mediate infection of cells lacking the primary receptor for JHM ("receptor-independent spread"). These enhanced fusogenic properties of viruses encoding a glycine at position 310 of the S protein may contribute to spread within the CNS, a tissue in which expression of conventional JHM receptors is low.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.