ArticleThe EMBO journal2003
A novel sensor of NADH/NAD+ redox poise in Streptomyces coelicolor A3(2).
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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Who cites it
112 citing papers in PubMed.
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- Reductive stress: The key pathway in metabolic disorders induced by overnutrition.Journal of advanced research · 2025Review
- AdpA, a Global Regulator of Hundreds of Genes, Including Those for Secondary Metabolism, inAntibiotics (Basel, Switzerland) · 2025Article
- Combined effect of polyphosphate kinase and lon protease in Streptomyces coelicolor A3(2) antibiotic production.Archives of microbiology · 2024Article
- Quantitative Galactose Colorimetric Competitive Assay Based on Galactose Dehydrogenase and Plasmonic Gold Nanostars.Biosensors · 2023Article
- Multi-omics reveal neuroprotection of Acer truncatum Bunge Seed extract on hypoxic-ischemia encephalopathy rats under high-altitude.Communications biology · 2023Article
- Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances inInternational journal of molecular sciences · 2022Article
- Article
- Structural Basis of Redox-Sensing Transcriptional Repressor Rex with Cofactor NADInternational journal of molecular sciences · 2022Article
- Genetic Circuit Design in Rhizobacteria.Biodesign research · 2022Review
- Effect of Divalent Metal Ion on the Structure, Stability and Function ofInternational journal of molecular sciences · 2021Article
- Bacterial approaches to sensing and responding to respiration and respiration metabolites.Molecular microbiology · 2021Review
- The redox-responsive transcriptional regulator Rex represses fermentative metabolism and is required for Listeria monocytogenes pathogenesis.PLoS pathogens · 2021Article
- NAD+ pool depletion as a signal for the Rex regulon involved in Streptococcus agalactiae virulence.PLoS pathogens · 2021Article
- Bacterial Oxidases of the CytochromeAntioxidants & redox signaling · 2021Review
- How Bacterial Redox Sensors Transmit Redox Signals via Structural Changes.Antioxidants (Basel, Switzerland) · 2021Review
- Genome-Wide Transcription Factor DNA Binding Sites and Gene Regulatory Networks inFrontiers in microbiology · 2021Article
- A Proteomic Analysis Indicates That Oxidative Stress Is the Common Feature Triggering Antibiotic Production inFrontiers in microbiology · 2021Article
52 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the identification of Rex, a novel redox-sensing repressor that appears to be widespread among Gram-positive bacteria. In Streptomyces coelicolor Rex binds to operator (ROP) sites located upstream of several respiratory genes, including the cydABCD and rex-hemACD operons. The DNA-binding activity of Rex appears to be controlled by the redox poise of the NADH/NAD+ pool. Using electromobility shift and surface plasmon resonance assays we show that NADH, but not NAD+, inhibits the DNA-binding activity of Rex. However, NAD+ competes with NADH for Rex binding, allowing Rex to sense redox poise over a range of NAD(H) concentrations. Rex is predicted to include a pyridine nucleotide-binding domain (Rossmann fold), and residues that might play key structural and nucleotide binding roles are highly conserved. In support of this, the central glycine in the signature motif (GlyXGlyXXGly) is shown to be essential for redox sensing. Rex homologues exist in most Gram-positive bacteria, including human pathogens such as Staphylococcus aureus, Listeria monocytogenes and Streptococcus pneumoniae.
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