ArticleThe EMBO journal2003
Rationally designed insulator-like elements can block enhancer action in vitro.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Combined analyses of RNA-sequence and Hi-C along with GWAS loci-A novel approach to dissect keloid disorder genetic mechanism.PLoS genetics · 2022Article
- Selective interactions between diverse STEs organize the ANT-C Hox cluster.Scientific reports · 2018Article
- Towards quantitative analysis of gene regulation by enhancers.Epigenomics · 2017Review
- Insights into Genome Architecture Deduced from the Properties of Short Lac Repressor-mediated DNA Loops.Biophysical reviews · 2016Article
- Chromatin boundary elements organize genomic architecture and developmental gene regulation in Drosophila Hox clusters.World journal of biological chemistry · 2016Review
- An Organizational Hub of Developmentally Regulated Chromatin Loops in the Drosophila Antennapedia Complex.Molecular and cellular biology · 2015Article
- Quantitation of interactions between two DNA loops demonstrates loop domain insulation in E. coli cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Chromatin loops as allosteric modulators of enhancer-promoter interactions.PLoS computational biology · 2014Article
- Surviving an identity crisis: a revised view of chromatin insulators in the genomics era.Biochimica et biophysica acta · 2014Review
- Effective blocking of the white enhancer requires cooperation between two main mechanisms suggested for the insulator function.PLoS genetics · 2013Article
- Distant activation of transcription: mechanisms of enhancer action.Molecular and cellular biology · 2012Review
- Internucleosomal interactions mediated by histone tails allow distant communication in chromatin.The Journal of biological chemistry · 2012Article
- Confocal fluorescence detected linear dichroism imaging of isolated human amyloid fibrils. Role of supercoiling.Journal of fluorescence · 2011Article
- Article
- Roles of DNA looping in enhancer-blocking activity.Biophysical journal · 2011Article
- Article
- Analysis of distant communication on defined chromatin templates in vitro.Methods in molecular biology (Clifton, N.J.) · 2009Article
- CTCF-dependent enhancer-blocking by alternative chromatin loop formation.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Enhancer-promoter communication is regulated by insulator pairing in a Drosophila model bigenic locus.Molecular and cellular biology · 2008Article
- Probability of the site juxtaposition determines the rate of protein-mediated DNA looping.Biophysical journal · 2007Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulators are DNA sequences that are likely to be involved in formation of chromatin domains, functional units of gene expression in eukaryotes. Insulators can form domain boundaries and block inappropriate action of regulatory elements (such as transcriptional enhancers) in eukaryotic nuclei. Using an in vitro system supporting enhancer action over a large distance, the enhancer-blocking insulator activity has been recapitulated in a highly purified system. The insulator-like element was constructed using a sequence-specific DNA-binding protein making stable DNA loops (lac repressor). The insulation was entirely dependent on formation of a DNA loop that topologically isolates the enhancer from the promoter. This rationally designed, inducible insulator-like element recapitulates many key properties of eukaryotic insulators observed in vivo. The data suggest novel mechanisms of enhancer and insulator action.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.