ArticleThe Biochemical journal2003
Class III phosphoinositide 3-kinase--Beclin1 complex mediates the amino acid-dependent regulation of autophagy in C2C12 myotubes.
Article in The Biochemical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 205 citations in OpenAlex.
- Molecular mechanisms of post-burn muscle wasting and the therapeutic potential of physical exercise.Journal of cachexia, sarcopenia and muscle · 2023Review
- Autophagy: A Key Regulator of Homeostasis and Disease: An Overview of Molecular Mechanisms and Modulators.Cells · 2022Review
- Autophagy Mediates MMP-2 Expression in Glaucomatous Trabecular Meshwork Cells.Journal of ophthalmology · 2022Article
- VPS38/UVRAG and ATG14, the variant regulatory subunits of the ATG6/Beclin1-PI3K complexes, are crucial for the biogenesis of the yolk organelles and are transcriptionally regulated in the oocytes of the vector Rhodnius prolixus.PLoS neglected tropical diseases · 2021Article
- Deciphering the Role of Autophagy in Treatment of Resistance Mechanisms in Glioblastoma.International journal of molecular sciences · 2021Review
- Mitophagy and the Brain.International journal of molecular sciences · 2020Review
- Review
- Article
- The Effect of Autophagic Activity on the Function of Apheresis Platelets and on the Efficacy of Clinical Platelet Transfusion.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2020Article
- Autophagy, apoptosis, and mitochondria: molecular integration and physiological relevance in skeletal muscle.American journal of physiology. Cell physiology · 2019Article
- Hyperoside decreases the apoptosis and autophagy rates of osteoblast MC3T3‑E1 cells by regulating TNF‑like weak inducer of apoptosis and the p38mitogen activated protein kinase pathway.Molecular medicine reports · 2019Article
- Article
- Influence of Hydrogen-rich Saline on Hepatocyte Autophagy During Laparoscopic Liver Ischaemia-reperfusion Combined Resection Injury in Miniature Pigs.Journal of veterinary research · 2018Article
- Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis.Journal of cellular and molecular medicine · 2018Article
- Decreased grip strength, muscle pain, and atrophy occur in rats following long-term exposure to excessive repetitive motion.FEBS open bio · 2017Article
- TMEM74 promotes tumor cell survival by inducing autophagy via interactions with ATG16L1 and ATG9A.Cell death & disease · 2017Article
- Role of Autophagy in HIV-1 Matrix Protein p17-Driven Lymphangiogenesis.Journal of virology · 2017Article
- Nuclear factor erythroid 2-related factor 2 enhances carcinogenesis by suppressing apoptosis and promoting autophagy in nickel-transformed cells.The Journal of biological chemistry · 2017Article
- Article
- FoxO1-AMPK-ULK1 Regulates Ethanol-Induced Autophagy in Muscle by Enhanced ATG14 Association with the BECN1-PIK3C3 Complex.Alcoholism, clinical and experimental research · 2017Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increased proteolysis contributes to muscle atrophy that prevails in many diseases. Elucidating the signalling pathways responsible for this activation is of obvious clinical importance. Autophagy is a ubiquitous degradation process, induced by amino acid starvation, that delivers cytoplasmic components to lysosomes. Starvation markedly stimulates autophagy in myotubes, and the present studies investigate the mechanisms of this regulation. In C(2)C(12) myotubes incubated with serum growth factors, amino acid starvation stimulated autophagic proteolysis independently of p38 and p42/p44 mitogen-activated protein kinases, but in a PI3K (phosphoinositide 3-kinase)-dependent manner. Starvation, however, did not alter activities of class I and class II PI3Ks, and was not sufficient to affect major signalling proteins downstream from class I PI3K (glycogen synthase kinase, Akt/protein kinase B and protein S6). In contrast, starvation increased class III PI3K activity in whole-myotube extracts. In fact, this increase was most pronounced for a population of class III PI3K that coimmunoprecipitated with Beclin1/Apg6 protein, a major determinant in the initiation of autophagy. Stimulation of proteolysis was reproduced by feeding myotubes with synthetic dipalmitoyl-PtdIns3 P, the class III PI3K product. Conversely, protein transfection of anti-class III PI3K inhibitory antibody into starved myotubes inverted the induction of proteolysis. Therefore, independently of class I PI3K/Akt, protein S6 and mitogen-activated protein kinase pathways, amino acid starvation stimulates proteolysis in myotubes by regulating class III PI3K-Beclin1 autophagic complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.