ArticleClinical and diagnostic laboratory immunology2003
Negative immunodiffusion test results obtained with sera of paracoccidioidomycosis patients may be related to low-avidity immunoglobulin G2 antibodies directed against carbohydrate epitopes.
Article in Clinical and diagnostic laboratory immunology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Standardization of Semi-Quantitative Dot Blotting Assay-Application in the Diagnosis, Follow-Up, and Relapse of Paracoccidioidomycosis.Microorganisms · 2024Article
- Evaluation of commercial immunodiffusion reagents for detecting serum anti-Paracoccidioides antibodies.Revista da Sociedade Brasileira de Medicina Tropical · 2024Article
- Immunologic Diagnosis of Endemic Mycoses.Journal of fungi (Basel, Switzerland) · 2022Review
- Effects of metaperiodate and urea solutions on the serological diagnosis of human sporotrichosis using an indirect ELISA test.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Case Report: Misleading Serological Diagnosis of Paracoccidioidomycosis in a Young Patient with the Acute Form Disease:The American journal of tropical medicine and hygiene · 2018Article
- Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis.PLoS neglected tropical diseases · 2017Article
- Paracoccidioides lutzii Plp43 is an active glucanase with partial antigenic identity with P. brasiliensis gp43.PLoS neglected tropical diseases · 2014Article
- Paracoccidioides brasiliensis and P. lutzii antigens elicit different serum IgG responses in chronic paracoccidioidomycosis.Mycopathologia · 2013Article
- Development and evaluation of a latex agglutination test for the serodiagnosis of paracoccidioidomycosis.Clinical and vaccine immunology : CVI · 2011Article
- Article
- Increased immunoglobulin G anti-Paracoccidioides brasiliensis serum antibody avidity as a predictor of favorable posttherapeutic evolution in paracoccidioidomycosis.Clinical and vaccine immunology : CVI · 2009Article
- Detection of Paracoccidioides brasiliensis gp43 gene in sputa by loop-mediated isothermal amplification method.Journal of clinical laboratory analysis · 2009Article
- Use of recombinant gp43 isoforms expressed in Pichia pastoris for diagnosis of paracoccidioidomycosis.Clinical and vaccine immunology : CVI · 2008Article
- Diagnosis of neuroparacoccidioidomycosis by detection of circulating antigen and antibody in cerebrospinal fluid.Journal of clinical microbiology · 2005Article
- Atypical serological response marked by a lack of detectable anti-gp43 antibodies in a patient with disseminated paracoccidioidomycosis.Journal of clinical microbiology · 2005Article
- Detection of Paracoccidioides brasiliensis gp70 circulating antigen and follow-up of patients undergoing antimycotic therapy.Journal of clinical microbiology · 2004Article
- Monitoring gp43 antigenemia in Paracoccidioidomycosis patients during therapy.Journal of clinical microbiology · 2004Article
- Detection of circulating gp43 antigen in serum, cerebrospinal fluid, and bronchoalveolar lavage fluid of patients with paracoccidioidomycosis.Journal of clinical microbiology · 2003Article
- Serology of paracoccidioidomycosis.MycopathologiaReview
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunodiffusion (ID) is the serologic test most frequently used for the diagnosis and posttherapy follow-up of patients with paracoccidioidomycosis (PCM). The ID test is highly specific (100%), but its sensitivity is relatively low (90%), leading to false-negative results. The aim of this study was to determine the profiles of antibodies in sera from patients with proven PCM and with negative results in the ID test (IDneg) versus positive results in the ID test (IDpos). We analyzed 46 sera from patients with active PCM for total immunoglobulin G (IgG) and IgG subclass responses to Paracoccidioides brasiliensis gp43 antigen (treated or not treated with sodium metaperiodate) by enzyme-linked immunosorbent assay and immunoblotting. Immunoblotting showed that both IDneg and IDpos sera recognized predominantly the gp43 fraction of the P. brasiliensis antigen used in the ID test. IDneg sera contain low-avidity antibodies, low levels of specific IgG (total) and IgG1, and high levels of IgG2 compared with IDpos sera. The antibodies present in IDneg sera were predominantly directed against carbohydrate epitopes, since treatment with sodium metaperiodate resulted in a significant decrease in antibody reactivity. These data suggest that the lack of reactivity of sera from PCM patients in the ID test may be related to the production of low-avidity IgG2 antibodies directed against carbohydrate epitopes.
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