ArticleGene therapy2003
Branched oligonucleotides induce in vivo gene conversion of a mutated EGFP reporter.
Article in Gene therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- The origin of unwanted editing byproducts in gene editing.Acta biochimica et biophysica Sinica · 2022Article
- Strand bias influences the mechanism of gene editing directed by single-stranded DNA oligonucleotides.Nucleic acids research · 2011Article
- Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides.Nucleic acids research · 2009Article
- DNA breakage associated with targeted gene alteration directed by DNA oligonucleotides.Mutation research · 2009Article
- Targeted gene knock in and sequence modulation mediated by a psoralen-linked triplex-forming oligonucleotide.The Journal of biological chemistry · 2008Article
- Simultaneous targeted exchange of two nucleotides by single-stranded oligonucleotides clusters within a region of about fourteen nucleotides.BMC molecular biology · 2008Article
- Article
- Manipulation of cell cycle progression can counteract the apparent loss of correction frequency following oligonucleotide-directed gene repair.BMC molecular biology · 2007Article
- Reduction of gene repair by selenomethionine with the use of single-stranded oligonucleotides.BMC molecular biology · 2007Article
- Gene therapy progress and prospects: targeted gene repair.Gene therapy · 2005Review
- Implications of cell cycle progression on functional sequence correction by short single-stranded DNA oligonucleotides.Gene therapy · 2005Article
- Stable transmission of targeted gene modification using single-stranded oligonucleotides with flanking LNAs.Nucleic acids research · 2005Article
- Correction of the neuropathogenic human apolipoprotein E4 (APOE4) gene to APOE3 in vitro using synthetic RNA/DNA oligonucleotides (chimeraplasts).Journal of molecular neuroscience : MN · 2005Article
- Camptothecin enhances the frequency of oligonucleotide-directed gene repair in mammalian cells by inducing DNA damage and activating homologous recombination.Nucleic acids research · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Branched oligonucleotides (b-oligonucleotides) based on a novel branching monomer were used for site-specific sequence alteration in vivo. With a stable integrated mutated enhanced green fluorescent protein (EGFP) template in Chinese hamster ovary cells, up to 0.1% EGFP-positive cells were counted after transfection with b-oligonucleotides. The presence of EGFP protein in converted cells was demonstrated by anti-EGFP immunocytochemistry. Genomic sequencing of converted cells showed in 40% of the analysed clones the corrected wild-type codon, while 9.3% of the sequences showed a corrected wild-type sequence and an additional collateral mutation. Despite the stable corrected genomic locus, converted cells entered selective apoptosis after 3-6 days. The cell line Irs-1 that is deficient in the homologous recombination pathway showed a reduced frequency of b-oligonucleotide-induced site-specific sequence conversion. The reduced conversion rates in the mutant cell line could be partly rescued by complementation with XRCC2 cDNA.
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Registered trials
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