ArticleNature biotechnology2003
Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors.
Article in Nature biotechnology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 60 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
60 citing papers in PubMed, 147 citations in OpenAlex.
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- Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology.Biomolecules · 2021Review
- Transcription factor stoichiometry in cell fate determination.Journal of genetics · 2021Review
- Functional Comparison between VP64-dCas9-VP64 and dCas9-VP192 CRISPR Activators in Human Embryonic Kidney Cells.International journal of molecular sciences · 2021Article
- Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories.Frontiers in bioengineering and biotechnology · 2020Review
- Engineering the Effector Domain of the Artificial Transcription Factor to Improve Cellulase Production byFrontiers in bioengineering and biotechnology · 2020Article
- Global Reprogramming of Gene Transcription inFrontiers in bioengineering and biotechnology · 2020Article
- Synthetic evolution.Nature biotechnology · 2019Review
- Use of fusion transcription factors to reprogram cellulase transcription and enable efficient cellulase production inBiotechnology for biofuels · 2019Article
- Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype.Biotechnology for biofuels · 2019Review
- Article
- Reprogramming cell fate with artificial transcription factors.FEBS letters · 2018Review
- Improved xylose tolerance and 2,3-butanediol production ofBiotechnology for biofuels · 2018Article
- Randomized CRISPR-Cas Transcriptional Perturbation Screening Reveals Protective Genes against Alpha-Synuclein Toxicity.Molecular cell · 2017Article
- Enhancement of Arabidopsis growth characteristics using genome interrogation with artificial transcription factors.PloS one · 2017Article
- ImprovingBiotechnology for biofuels · 2017Article
- Reprogramming cell fate with a genome-scale library of artificial transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
- Erratum issuedLee, Sung-Il [corrected to Lee, Seong-il]
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a method in which randomized libraries of zinc finger-containing artificial transcription factors are used to induce phenotypic variations in yeast and mammalian cells. By linking multiple zinc-finger domains together, we constructed more than 100,000 zinc-finger proteins with diverse DNA-binding specificities and fused each of them to either a transcription activation or repression domain. The resulting transcriptional regulatory proteins were expressed individually in cells, and the transfected cells were screened for various phenotypic changes, such as drug resistance, thermotolerance or osmotolerance in yeast, and differentiation in mammalian cells. Genes associated with the selected phenotypes were also identified. Our results show that randomized libraries of artificial transcription factors are useful tools for functional genomics and phenotypic engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.