ArticleMolecular biology of the cell2003
Recycling of Raft-associated prohormone sorting receptor carboxypeptidase E requires interaction with ARF6.
Article in Molecular biology of the cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Rab3 mediates a pathway for endocytic sorting and plasma membrane recycling of ordered microdomains.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Trafficking of hormones and trophic factors to secretory and extracellular vesicles: a historical perspective and new hypothesis.Extracellular vesicles and circulating nucleic acids · 2023Article
- Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP.Molecular biology of the cell · 2018Article
- Article
- BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells.The Journal of cell biology · 2017Article
- Dissecting carboxypeptidase E: properties, functions and pathophysiological roles in disease.Endocrine connections · 2017Review
- 60 YEARS OF POMC: Biosynthesis, trafficking, and secretion of pro-opiomelanocortin-derived peptides.Journal of molecular endocrinology · 2016Review
- New roles of carboxypeptidase E in endocrine and neural function and cancer.Endocrine reviews · 2012Review
- Exploring the membrane topology of prohormone convertase 1 in AtT20 Cells: in situ analysis by immunofluorescence microscopy.F1000Research · 2012Article
- Carboxypeptidase E cytoplasmic tail mediates localization of synaptic vesicles to the pre-active zone in hypothalamic pre-synaptic terminals.Journal of neurochemistry · 2010Article
- Endocytosis unplugged: multiple ways to enter the cell.Cell research · 2010Review
- CD82 endocytosis and cholesterol-dependent reorganization of tetraspanin webs and lipid rafts.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Clathrin-independent endocytosis: a unique platform for cell signaling and PM remodeling.Cellular signalling · 2009Review
- How peptide hormone vesicles are transported to the secretion site for exocytosis.Molecular endocrinology (Baltimore, Md.) · 2008Review
- A bi-directional carboxypeptidase E-driven transport mechanism controls BDNF vesicle homeostasis in hippocampal neurons.Molecular and cellular neurosciences · 2008Article
- A transient forward-targeting element for microneme-regulated secretion in Toxoplasma gondii.Biology of the cell · 2008Article
- Carboxypeptidase E cytoplasmic tail-driven vesicle transport is key for activity-dependent secretion of peptide hormones.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity.Molecular and cellular biology · 2007Article
- Pathways of clathrin-independent endocytosis.Nature reviews. Molecular cell biology · 2007Review
- Clathrin- and caveolin-1-independent endocytosis: entry of simian virus 40 into cells devoid of caveolae.The Journal of cell biology · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Little is known about the molecular mechanism of recycling of intracellular receptors and lipid raft-associated proteins. Here, we have investigated the recycling pathway and internalization mechanism of a transmembrane, lipid raft-associated intracellular prohormone sorting receptor, carboxypeptidase E (CPE). CPE is found in the trans-Golgi network (TGN) and secretory granules of (neuro)endocrine cells. An extracellular domain of the IL2 receptor alpha-subunit (Tac) fused to the transmembrane domain and cytoplasmic tail of CPE (Tac-CPE25) was used as a marker to track recycling of CPE. We show in (neuro)endocrine cells, that upon stimulated secretory granule exocytosis, raft-associated Tac-CPE25 was rapidly internalized from the plasma membrane in a clathrin-independent manner into early endosomes and then transported through the endocytic recycling compartment to the TGN. A yeast two-hybrid screen and in vitro binding assay identified the CPE cytoplasmic tail sequence S472ETLNF477 as an interactor with active small GTPase ADP-ribosylation factor (ARF) 6, but not ARF1. Expression of a dominant negative, inactive ARF6 mutant blocked this recycling. Mutation of residues S472 or E473 to A in the cytoplasmic tail of CPE obliterated its binding to ARF6, and internalization from the plasma membrane of Tac-CPE25 mutated at S472 or E473 was significantly reduced. Thus, CPE recycles back to the TGN by a novel mechanism requiring ARF6 interaction and activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.