ArticleProceedings of the National Academy of Sciences of the United States of America2003
Rho-dependent formation of epithelial "leader" cells during wound healing.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed, 235 citations in OpenAlex.
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- Regulation of corneal stromal cell behavior by modulating curvature using a hydraulically-controlled organ chip array.Nature communications · 2025Article
- Activation of a Src-JNK pathway in unscheduled endocycling cells of the Drosophila wing disc induces a chronic wounding response.Genetics · 2025Article
- Galvanotactic directionality of cell groups depends on group size.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Regulation of cell migration by extracellular matrix mechanics at a glance.Journal of cell science · 2025Review
- Geometrical constraint change determines organized collective migration of follower cells.Scientific reports · 2025Article
- Recent advances in the role of neuroregulation in skin wound healing.Burns & trauma · 2025Review
- Fast yet force-effective mode of supracellular collective cell migration due to extracellular force transmission.PLoS computational biology · 2025Article
- Leader Cells: Invade and Evade-The Frontline of Cancer Progression.International journal of molecular sciences · 2024Review
- The Role of TGF-β1 and MutantCancers · 2024Article
- Modeling the roles of cohesotaxis, cell-intercalation, and tissue geometry in collective cell migration of Xenopus mesendoderm.Biology open · 2024Article
- PIEZO1 regulates leader cell formation and cellular coordination during collective keratinocyte migration.PLoS computational biology · 2024Article
- A life off the beaten track in biomechanics: Imperfect elasticity, cytoskeletal glassiness, and epithelial unjamming.Biophysics reviews · 2023Review
- A planar polarized MYO6-DOCK7-RAC1 axis promotes tissue fluidification in mammary epithelia.Cell reports · 2023Article
- Cellular mechanisms of wound closure under cyclic stretching.Biophysical journal · 2023Article
- Spatially Defined Cell-Secreted Protein Detection Using Granular Hydrogels: μGeLISA.ACS biomaterials science & engineering · 2023Article
- Electric field-guided collective motility initiation of large epidermal cell groups.Molecular biology of the cell · 2023Article
- Imaging and detecting intercellular tensile forces in spheroids and embryoid bodies using lipid-modified DNA probes.Frontiers in cell and developmental biology · 2023Article
53 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The motile behavior of epithelial cells located at the edge of a large wound in a monolayer of cultured cells was analyzed. The initial cellular response is alignment of the edge with an accompanying formation of tangential marginal actin bundles within individual cells positioned along the wound edge. Later, coherent out-growths of cell masses occur by the formation of special "leader" cells at the tops of outgrowths and "follower" cells along the sides. Leader cells exhibit profound cytoskeletal reorganization, including disassembly of marginal bundles, the realignment of actin filament bundles, and penetration of microtubules into highly active lamellae. Additionally, cell-cell contacts acquire radial geometry indicative of increased contractile tension. Interestingly, leader cells acquire a cytoskeletal organization and motility typical of fibroblasts. IAR-2 cultures stably transfected with a dominant-negative mutant of RhoA or treated with Rho-kinase inhibitor Y-27632 transformed most edge cells into leader-like cells. Alternatively, transfection of cells with constitutively active RhoA suppressed formation of leaders. Thus, expansion of the epithelial sheet involves functional differentiation into two distinct types of edge cells. The transition between these two patterns is controlled by Rho activity, which in turn controls the dynamic distribution and activity of actin filament bundles, myosin II, and microtubules.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.