ArticleProceedings of the National Academy of Sciences of the United States of America2003
Nuclear actin and protein 4.1: essential interactions during nuclear assembly in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 101 citations in OpenAlex.
- Nuclear actin filaments - a historical perspective.Nucleus (Austin, Tex.) · 2024Review
- FERM domain-containing proteins are active components of the cell nucleus.Life science alliance · 2024Review
- Nuclear F-actin and Lamin A antagonistically modulate nuclear shape.Journal of cell science · 2022Article
- The Role of Emerin in Cancer Progression and Metastasis.International journal of molecular sciences · 2021Review
- Review
- New Insights into Cellular Functions of Nuclear Actin.Biology · 2021Review
- Loss of 4.1N in epithelial ovarian cancer results in EMT and matrix-detached cell death resistance.Protein & cell · 2021Article
- Multiple Pools of Nuclear Actin.Anatomical record (Hoboken, N.J. : 2007) · 2018Article
- Baculovirus Actin-Based Motility Drives Nuclear Envelope Disruption and Nuclear Egress.Current biology : CB · 2018Article
- C3G dynamically associates with nuclear speckles and regulates mRNA splicing.Molecular biology of the cell · 2018Article
- The Nature of Actin-Family Proteins in Chromatin-Modifying Complexes.Frontiers in genetics · 2018Review
- Initiation of DNA replication requires actin dynamics and formin activity.The EMBO journal · 2017Article
- Nuclear speckles: molecular organization, biological function and role in disease.Nucleic acids research · 2017Review
- Nuclear Actin in Development and Transcriptional Reprogramming.Frontiers in genetics · 2017Review
- Protein 4.1R Influences Myogenin Protein Stability and Skeletal Muscle Differentiation.The Journal of biological chemistry · 2016Article
- Diseases of the Nucleoskeleton.Comprehensive Physiology · 2016Review
- Actin, actin-binding proteins, and actin-related proteins in the nucleus.Histochemistry and cell biology · 2016Review
- Protein 4.1G Regulates Cell Adhesion, Spreading, and Migration of Mouse Embryonic Fibroblasts through the β1 Integrin Pathway.The Journal of biological chemistry · 2016Article
- Nuclear actin and myosins in adenovirus infection.Experimental cell research · 2015Article
- Involvement of the SATB1/F-actin complex in chromatin reorganization during active cell death.International journal of molecular medicine · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Structural protein 4.1, which has crucial interactions within the spectrin-actin lattice of the human red cell membrane skeleton, also is widely distributed at diverse intracellular sites in nucleated cells. We previously showed that 4.1 is essential for assembly of functional nuclei in vitro and that the capacity of 4.1 to bind actin is required. Here we report that 4.1 and actin colocalize in mammalian cell nuclei using fluorescence microscopy and, by higher-resolution detergent-extracted cell whole-mount electron microscopy, are associated on nuclear filaments. We also devised a cell-free assay using Xenopus egg extract containing fluorescent actin to follow actin during nuclear assembly. By directly imaging actin under nonperturbing conditions, the total nuclear actin population is retained and visualized in situ relative to intact chromatin. We detected actin initially when chromatin and nuclear pores began assembling. As nuclear lamina assembled, but preceding DNA synthesis, actin distributed in a reticulated pattern throughout the nucleus. Protein 4.1 epitopes also were detected when actin began to accumulate in nuclei, producing a diffuse coincident pattern. As nuclei matured, actin was detected both coincident with and also independent of 4.1 epitopes. To test whether acquisition of nuclear actin is required for nuclear assembly, the actin inhibitor latrunculin A was added to Xenopus egg extracts during nuclear assembly. Latrunculin A strongly perturbed nuclear assembly and produced distorted nuclear structures containing neither actin nor protein 4.1. Our results suggest that actin as well as 4.1 is necessary for nuclear assembly and that 4.1-actin interactions may be critical.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.