Evidence map›Paper›PMID 12958287›Full record

ArticleJournal of clinical microbiology2003

Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus.

Akihiro Yoshida, Nao Suzuki, Yoshio Nakano, Miki Kawada, Takahiko Oho, Toshihiko Koga

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 152 citations in OpenAlex.

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  10. OralFrontiers in dental medicine · 2025
    Article
  11. qPCR assay optimisation for a clinical study comparing oral health risk in Rett syndrome.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2024
    Article
  12. Article
  13. Article
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  17. Ecological influence by colonization of fluoride-resistantFrontiers in cellular and infection microbiology · 2022
    Article
  18. Article
  19. Article
  20. Article

11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Akihiro YoshidaDepartment of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
Nao Suzuki
Yoshio Nakano
Miki Kawada
Takahiko Oho
Toshihiko Koga
Kyushu Dental University · JPKyushu University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.

Indexed as

BiofilmsDental CariesDental PlaqueHumansPolymerase Chain ReactionSalivaStreptococcus mutansStreptococcus sobrinusTaq PolymeraseTaq Polymerase

Identifiers

PMID12958287
PMCPMC193796
OpenAlexW2127466890

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.