ArticleJournal of clinical microbiology2003
GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 125 citations in OpenAlex.
- Effectiveness of a Behavior Change Intervention with Hand Sanitizer Use and Respiratory Hygiene in Reducing Laboratory-Confirmed Influenza among Schoolchildren in Bangladesh: A Cluster Randomized Controlled Trial.The American journal of tropical medicine and hygiene · 2019Trial
- Genetic analysis of the 2019 coronavirus pandemic with from real-time reverse transcriptase polymerase chain reaction.Saudi journal of biological sciences · 2021Review
- Advances in laboratory assays for detecting human metapneumovirus.Annals of translational medicine · 2020Review
- Viral etiology of pneumonia among severely malnourished under-five children in an urban hospital, Bangladesh.PloS one · 2020Article
- The Impact of RSV-Associated Respiratory Disease on Children in Asia.Journal of pediatric infectious diseases · 2019Review
- Bacterial load and inflammatory response in sputum of alpha-1 antitrypsin deficiency patients with COPD.International journal of chronic obstructive pulmonary disease · 2019Article
- Respiratory Viruses and Atypical Bacteria Co-Infection in Children with Acute Respiratory Infection.Open access Macedonian journal of medical sciences · 2018Article
- Incidence and etiology of hospitalized acute respiratory infections in the Egyptian Delta.Influenza and other respiratory viruses · 2017Article
- Hospitalizations and outpatient visits for rhinovirus-associated acute respiratory illness in adults.The Journal of allergy and clinical immunology · 2016Article
- Viral aetiology of common colds of outpatient children at primary care level and the use of antibiotics.Memorias do Instituto Oswaldo Cruz · 2015Observational
- Degenerate PCR primer design for the specific identification of rhinovirus C.Journal of virological methods · 2015Article
- Epidemiological and clinical evaluation of children with respiratory virus infections.Medical journal of the Islamic Republic of Iran · 2014Article
- Is there still room for novel viral pathogens in pediatric respiratory tract infections?PloS one · 2014Article
- Application of three duplex real-time PCR assays for simultaneous detection of human seasonal and avian influenza viruses.Archives of virology · 2013Article
- Hospitalization due to human parainfluenza virus-associated lower respiratory tract illness in rural Thailand.Influenza and other respiratory viruses · 2013Article
- A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis.Memorias do Instituto Oswaldo Cruz · 2013Article
- Comparison of nasopharyngeal flocked swabs and nasopharyngeal wash collection methods for respiratory virus detection in hospitalized children using real-time polymerase chain reaction.Journal of virological methods · 2012Article
- Viral agents causing lower respiratory tract infections in hospitalized children: evaluation of the Speed-Oligo® RSV assay for the detection of respiratory syncytial virus.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Human coronavirus NL-63 infection in a Brazilian patient suspected of H1N1 2009 influenza infection: description of a fatal case.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
- Respiratory viruses involved in influenza-like illness in a Greek pediatric population during the winter period of the years 2005-2008.Journal of medical virology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was developed to detect six common respiratory viruses in clinical specimens from young children. Assays for human respiratory syncytial virus (HRSV); human parainfluenza viruses 1, 2, and 3 (HPIV1, -2, and -3, respectively); and influenza A and B viruses were incorporated into a single standard assay format. The optimized assay panel was used to test 470 respiratory specimens obtained from 462 children hospitalized with acute respiratory illness that had been previously tested by viral culture (405 specimens) or direct immunofluorescence staining (DIF) (65 specimens). Of 93 specimens positive for respiratory viruses by culture or DIF, 86 (92%) were positive by RT-PCR, including 66 HRSV, 2 HPIV2, 5 HPIV3, 3 influenza A virus, and 10 influenza B virus specimens. An additional 119 respiratory viruses were identified by RT-PCR in 116 patients for whom results were negative by viral isolation or DIF. We conclude that the GeneScan RT-PCR panel can markedly improve detection of acute respiratory virus infections in young children.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.