ArticleJournal of clinical microbiology2003
Intraspecies genotype variability of the microsporidian parasite Encephalitozoon hellem.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Influence of host cell line and microsporidian species in the in vitro infection efficiency of Encephalitozoon spp.Parasite (Paris, France) · 2026Article
- Detection and Genetic Characterization ofAnimals : an open access journal from MDPI · 2025Article
- Novel zoonoticCurrent research in parasitology & vector-borne diseases · 2025Article
- Wildlife as Reservoirs of Encephalitozoon Cuniculi and Encephalitozoon Hellem and Molecular Genotyping of Encephalitozoon spp. in Small Mammals in the Czech Republic.Acta parasitologica · 2024Article
- Case series of intestinal microsporidiosis in non-HIV patients caused byEmerging microbes & infections · 2023Article
- The microsporidian polar tube: origin, structure, composition, function, and application.Parasites & vectors · 2023Review
- High Prevalence of Microsporidia in the North African Hedgehog (Animals : an open access journal from MDPI · 2023Article
- Intraspecific genetic diversity of the fish-infecting microsporidian parasiteFrontiers in microbiology · 2023Article
- First report ofParasitology · 2022Article
- Chronic Infections in Mammals Due to Microsporidia.Experientia supplementum (2012) · 2022Article
- The Function and Structure of the Microsporidia Polar Tube.Experientia supplementum (2012) · 2022Article
- Are molecular tools clarifying or confusing our understanding of the public health threat from zoonotic enteric protozoa in wildlife?International journal for parasitology. Parasites and wildlife · 2019Review
- Molecular diagnosis of microsporidia strains in slaughtered cows of southwest of Iran.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2018Article
- Genetic diversity of two Daphnia-infecting microsporidian parasites, based on sequence variation in the internal transcribed spacer region.Parasites & vectors · 2016Article
- More than a rabbit's tale -International journal for parasitology. Parasites and wildlife · 2016Review
- Cloning and sequencing of beta-tubulin and internal transcribed spacer-2 (ITS-2) of Eimeria tenella isolate from India.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2015Article
- Detection of Encephalitozoon spp. from human diarrheal stool and farm soil samples in Korea.Journal of Korean medical science · 2015Article
- Complete genome sequences from three genetically distinct strains reveal high intraspecies genetic diversity in the microsporidian Encephalitozoon cuniculi.Eukaryotic cell · 2013Article
- Identification and characterization of microsporidia from fecal samples of HIV-positive patients from Lagos, Nigeria.PloS one · 2012Article
- Unapparent microsporidial infection among immunocompetent humans in the Czech Republic.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seven isolates of Encephalitozoon hellem from human immunodeficiency virus-positive patients were genotyped through a series of markers: the internal transcribed spacer (ITS) of ribosomal DNA, the polar tube protein (PTP) gene, and two intergenic spacers (IGS-TH and IGS-HZ) whose polymorphism is newly reported. The genome markers were all analyzed at three levels: PCR amplification followed by polyacrylamide gel electrophoresis, single-strand conformation analysis (SSCA), and DNA sequencing. The polymorphisms detected involve insertions/deletions and point mutations. SSCA can distinguish any pair of sequences, even those differing by a single base pair. The different isolates studied fit into the previously described ITS genotype 1A, except one which seems to be a 2A derivative variant (2D). When PTP and the new markers IGS-TH and IGS-HZ were analyzed, most of the isolates displayed different genotypes, demonstrating that E. hellem has a strong intraspecies variability. A set of markers such as those used here may be very useful in genotyping of clinical samples and in the assessment of epidemiological relationships among E. hellem strains.
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