ArticleJournal of clinical microbiology2003
Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 126 citations in OpenAlex.
- Emergence of Bordetella holmesii-Associated Pertussis-Like Illness, Northern India, 2019-2023.Emerging infectious diseases · 2025Article
- Global, regional, and national burdens of pertussis among adults: a systematic analysis of age-specific trends using Global Burden of Diseases 2021 data.Infectious diseases of poverty · 2025Article
- DecipheringJournal of clinical microbiology · 2024Article
- A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis.Scientific reports · 2023Article
- Mucosal Immunization with DTaP Confers Protection againstInfection and immunity · 2021Article
- Development and Validation of Two Diagnostic Real-Time PCR (TaqMan) Assays for the Detection ofMicroorganisms · 2021Article
- Evaluation of the Genmark ePlex® and QIAstat-Dx® respiratory pathogen panels in detecting bacterial targets in lower respiratory tract specimens.BMC microbiology · 2021Article
- Alarm Thresholds for Pertussis Outbreaks in Iran: National Data Analysis.Osong public health and research perspectives · 2020Article
- Multicenter Evaluation of QIAstat-Dx Respiratory Panel V2 for Detection of Viral and Bacterial Respiratory Pathogens.Journal of clinical microbiology · 2020Article
- Ellipsometric-based novel DNA biosensor for label-free, real-time detection of Bordetella parapertussis.Journal of biological physics · 2019Article
- A low-cost microfluidic platform for rapid and instrument-free detection of whooping cough.Analytica chimica acta · 2019Article
- Validation and Implementation of a Diagnostic Algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B. holmesii in a Pediatric Referral Hospital in Barcelona, Spain.Journal of clinical microbiology · 2019Article
- Clinical implications of rapid ePlex® Respiratory Pathogen Panel testing compared to laboratory-developed real-time PCR.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens.Journal of clinical microbiology · 2017Article
- Improving specificity of Bordetella pertussis detection using a four target real-time PCR.PloS one · 2017Article
- Comparing the Yield of Nasopharyngeal Swabs, Nasal Aspirates, and Induced Sputum for Detection of Bordetella pertussis in Hospitalized Infants.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Draft Genome Sequence of a Bordetella pertussis Strain with the Virulence-Associated Allelic Variant ptxP3, Isolated in Italy.Genome announcements · 2015Article
- Comparative evaluation of the Diagenode multiplex PCR assay on the BD max system versus a routine in-house assay for detection of Bordetella pertussis.Journal of clinical microbiology · 2014Article
- Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.Iranian journal of microbiology · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR is increasingly being used as a diagnostic test for the detection of Bordetella pertussis and Bordetella parapertussis DNA, as it has improved sensitivity and specificity in comparison to conventional techniques. The assay described here uses the two insertion sequences IS481 and IS1001 for B. pertussis and B. parapertussis, respectively, with detection by molecular beacons. The real-time PCR for IS481 detects both B. pertussis and Bordetella holmesii, and the real-time PCR for IS1001 detects both B. parapertussis and B. holmesii. By performing both assays discrimination between B. pertussis and B. parapertussis can be obtained. The sensitivity was 1 to 10 CFU/ml for B. pertussis, 10 CFU/ml for B. parapertussis, and 10 CFU/ml for B. holmesii in both assays. The clinical sensitivity of the B. pertussis assay was not affected by duplexing with an internal control PCR. Real-time PCR, conventional PCR, and culture were performed on 57 clinical samples. Eight of the 57 (14%) were found positive by culture, 19 of 57 (33%) were found positive by conventional PCR, and 22 of 57 (39%) were found positive by real-time PCR. One sample was inhibitory. When the B. pertussis assay was compared with a clinical standard for B. pertussis infection, sensitivity was 38, 83, and 100% and specificity was 100, 97, and 97% for culture, conventional PCR, and real-time PCR, respectively. The real-time PCR designed for B. pertussis and B. parapertussis provides sensitive and specific diagnosis of B. pertussis and B. parapertussis infections and is therefore suitable for implementation in the diagnostic laboratory.
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