ArticleJournal of clinical microbiology2003
Evaluation of a multiplex PCR test for simultaneous identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Serotyping ofMicrobial genomics · 2025Article
- Temporal and Serotypic Dynamics ofPathogens (Basel, Switzerland) · 2024Article
- Review
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- Explorative Field Study on the Use of Oral Fluids for the Surveillance ofVeterinary sciences · 2022Article
- Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae.MicrobiologyOpen · 2022Article
- Sero- and apx-typing of German Actinobacillus pleuropneumoniae field isolates from 2010 to 2019 reveals a predominance of serovar 2 with regular apx-profile.Veterinary research · 2021Article
- Coinfections and Phenotypic Antimicrobial Resistance inFrontiers in veterinary science · 2021Article
- Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.Veterinary microbiology · 2018Article
- Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis.Veterinary microbiology · 2018Article
- Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil.PloS one · 2018Article
- Etiology of acute respiratory disease in fattening pigs in Finland.Porcine health management · 2017Article
- The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14.The Journal of veterinary medical science · 2015Article
- The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 15.The Journal of veterinary medical science · 2015Article
- Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.Journal of clinical microbiology · 2014Article
- Isolation of atypical genotype Actinobacillus pleuropneumoniae serotype 6 in Japan.The Journal of veterinary medical science · 2014Article
- Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: A new insight into an old approach.Genetics and molecular biology · 2013Article
- Prevalence of Actinobacillus pleuropneumoniae serovars in England and Wales.The Veterinary record · 2010Article
- DNA microarray-based identification and typing of Actinobacillus pleuropneumoniae.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2009Article
- Multiplex PCR that can distinguish between immunologically cross- reactive serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains.Journal of clinical microbiology · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Serotype-specific DNA regions involved in the biosynthesis of capsular polysaccharides (cps region) were used to develop a multiplex PCR test for the simultaneous species identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6. Primers specific for serotypes 2, 5, and 6 were combined with the already existing species-specific primers used in a PCR test based on the omlA gene. The PCR test was evaluated with serotype reference strains of A. pleuropneumoniae as well as 182 Danish field isolates previously serotyped by latex agglutination or immunodiffusion. For all serologically typeable strains, a complete correspondence was found between the results obtained by the multiplex PCR test and the results obtained by the traditional serotyping methods. Six of eight serologically nontypeable strains could be allocated to a serotype on the basis of the multiplex PCR results. The species specificity of the assay was evaluated with a collection of 93 strains representing 29 different species within the family Pasteurellaceae, as well as species normally found in the respiratory tracts of swine. All of these strains were negative by the multiplex PCR test, including 50 field isolates of the phylogenetically closely related species Actinobacillus lignieresii. When the multiplex PCR test was used to test Danish field strains, it was able to identify the serotypes of approximately 94% of all strains isolated from swine with clinical disease. More than 90% of the isolates that cross-reacted by the latex agglutination test were of serotype 2, 5, or 6. Determination of the serotype by PCR represents a convenient and specific method for the serotyping of A. pleuropneumoniae in diagnostic laboratories.
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