ArticleApplied and environmental microbiology2003
Toward an international standard for PCR-based detection of food-borne thermotolerant Campylobacters: assay development and analytical validation.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Detection and characterization of Campylobacter in air samples from poultry houses using shot-gun metagenomics - a pilot study.BMC microbiology · 2024Article
- Polymerase Chain Reaction: A Better Method for Diagnosing Chronic Schistosoma mansoni Infections.Tropical medicine and health · 2015Article
- Environmental monitoring of waterborne Campylobacter: evaluation of the Australian standard and a hybrid extraction-free MPN-PCR method.Frontiers in microbiology · 2015Article
- "Limits of control"--crucial parameters for a reliable quantification of viable campylobacter by real-time PCR.PloS one · 2014Article
- Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment.Applied and environmental microbiology · 2010Article
- Thermotolerant coliforms are not a good surrogate for Campylobacter spp. in environmental water.Applied and environmental microbiology · 2009Article
- Plastic-adherent DNA aptamer-magnetic bead and quantum dot sandwich assay for Campylobacter detection.Journal of fluorescence · 2009Article
- Development of cpn60-based real-time quantitative PCR assays for the detection of 14 Campylobacter species and application to screening of canine fecal samples.Applied and environmental microbiology · 2009Article
- Detection of Campylobacter bacteria in air samples for continuous real-time monitoring of Campylobacter colonization in broiler flocks.Applied and environmental microbiology · 2009Article
- Multiplex PCR for simultaneous detection of bacteria of the genus Listeria, Listeria monocytogenes, and major serotypes and epidemic clones of L. monocytogenes.Applied and environmental microbiology · 2007Article
- Development of a multiplex real-time PCR assay with an internal amplification control for the detection of total and pathogenic Vibrio parahaemolyticus bacteria in oysters.Applied and environmental microbiology · 2007Article
- Comparative, collaborative, and on-site validation of a TaqMan PCR method as a tool for certified production of fresh, campylobacter-free chickens.Applied and environmental microbiology · 2006Article
- Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR.Applied and environmental microbiology · 2006Article
- Quantification of Campylobacter spp. in chicken rinse samples by using flotation prior to real-time PCR.Applied and environmental microbiology · 2005Article
- Detection of Campylobacter spp. in chicken fecal samples by real-time PCR.Journal of clinical microbiology · 2004Article
- Validation of a PCR-based method for detection of food-borne thermotolerant campylobacters in a multicenter collaborative trial.Applied and environmental microbiology · 2004Article
- Enrichment followed by quantitative PCR both for rapid detection and as a tool for quantitative risk assessment of food-borne thermotolerant campylobacters.Applied and environmental microbiology · 2004Article
- Practical considerations in design of internal amplification controls for diagnostic PCR assays.Journal of clinical microbiology · 2004Review
- Validated 5' nuclease PCR assay for rapid identification of the genus Brucella.Journal of clinical microbiology · 2004Article
- Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni.Folia microbiologica · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As part of a European research project (FOOD-PCR), we developed a standardized and robust PCR detection assay specific for the three most frequently reported food-borne pathogenic Campylobacter species, C. jejuni, C. coli, and C. lari. Fifteen published and unpublished PCR primers targeting the 16S rRNA gene were tested in all possible pairwise combinations, as well as two published primers targeting the 23S rRNA gene. A panel of 150 strains including target and nontarget strains was used in an in-house validation. Only one primer pair, OT1559 plus 18-1, was found to be selective. The inclusivity and exclusivity were 100 and 97%, respectively. In an attempt to find a thermostable DNA polymerase more resistant than Taq to PCR inhibitors present in chicken samples, three DNA polymerases were evaluated. The DNA polymerase Tth was not inhibited at a concentration of 2% (vol/vol) chicken carcass rinse, unlike both Taq DNA polymerase and DyNAzyme. Based on these results, Tth was selected as the most suitable enzyme for the assay. The standardized PCR test described shows potential for use in large-scale screening programs for food-borne Campylobacter species under the assay conditions specified.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.