ArticleApplied and environmental microbiology2003
Characterization of a theta-type plasmid from Lactobacillus sakei: a potential basis for low-copy-number vectors in lactobacilli.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- Characterization and sequencing analysis of pLP2.5-11 and pLP3.0-4 novel cryptic plasmids from3 Biotech · 2023Article
- Characterization of a novel theta-type replicon of indigenous plasmid pTE15 from Lactobacillus reuteri N16.BMC microbiology · 2022Article
- Recent progress on n-butanol production by lactic acid bacteria.World journal of microbiology & biotechnology · 2021Review
- Heme Uptake inApplied and environmental microbiology · 2020Article
- Nucleotide sequence and analysis of pRC12 and pRC18, two theta-replicating plasmids harbored by Lactobacillus curvatus CRL 705.PloS one · 2020Article
- Dextransucrase Expression Is Concomitant with that of Replication and Maintenance Functions of the pMN1 Plasmid inFrontiers in microbiology · 2017Article
- Isolation of a minireplicon of the plasmid pG6303 of Lactobacillus plantarum G63 and characterization of the plasmid-encoded Rep replication protein.Journal of genetics · 2015Article
- Catabolism of N-acetylneuraminic acid, a fitness function of the food-borne lactic acid bacterium Lactobacillus sakei, involves two newly characterized proteins.Applied and environmental microbiology · 2013Article
- Use of a novel Escherichia coli-leuconostoc shuttle vector for metabolic engineering of Leuconostoc citreum to overproduce D-lactate.Applied and environmental microbiology · 2013Article
- Alternative sigma factor σH activates competence gene expression in Lactobacillus sakei.BMC microbiology · 2012Article
- Comparative genomics of Lactobacillus sakei with emphasis on strains from meat.Molecular genetics and genomics : MGG · 2011Article
- Behavior of the meat-borne bacterium Lactobacillus sakei during its transit through the gastrointestinal tracts of axenic and conventional mice.Applied and environmental microbiology · 2009Article
- Induction of immune responses in mice after oral immunization with recombinant Lactobacillus casei strains expressing enterotoxigenic Escherichia coli F41 fimbrial protein.Applied and environmental microbiology · 2009Article
- Intraspecies genomic diversity and natural population structure of the meat-borne lactic acid bacterium Lactobacillus sakei.Applied and environmental microbiology · 2009Article
- Characterization of a rolling-circle replication plasmid pLR1 from Lactobacillus plantarum LR1.Current microbiology · 2009Article
- Characterization of a cryptic plasmid from a Greenland ice core Arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high G+C Gram-positive recipients.Extremophiles : life under extreme conditions · 2008Article
- Construction of plasmid vectors for screening replicons from gram-positive bacteria and their use as shuttle cloning vectors.Current microbiology · 2007Article
- Identification of Lactobacillus sakei genes induced during meat fermentation and their role in survival and growth.Applied and environmental microbiology · 2007Article
- The SXT conjugative element and linear prophage N15 encode toxin-antitoxin-stabilizing systems homologous to the tad-ata module of the Paracoccus aminophilus plasmid pAMI2.Journal of bacteriology · 2007Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The complete nucleotide sequence of the 13-kb plasmid pRV500, isolated from Lactobacillus sakei RV332, was determined. Sequence analysis enabled the identification of genes coding for a putative type I restriction-modification system, two genes coding for putative recombinases of the integrase family, and a region likely involved in replication. The structural features of this region, comprising a putative ori segment containing 11- and 22-bp repeats and a repA gene coding for a putative initiator protein, indicated that pRV500 belongs to the pUCL287 subfamily of theta-type replicons. A 3.7-kb fragment encompassing this region was fused to an Escherichia coli replicon to produce the shuttle vector pRV566 and was observed to be functional in L. sakei for plasmid replication. The L. sakei replicon alone could not support replication in E. coli. Plasmid pRV500 and its derivative pRV566 were determined to be at very low copy numbers in L. sakei. pRV566 was maintained at a reasonable rate over 20 generations in several lactobacilli, such as Lactobacillus curvatus, Lactobacillus casei, and Lactobacillus plantarum, in addition to L. sakei, making it an interesting basis for developing vectors. Sequence relationships with other plasmids are described and discussed.
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