ArticleApplied and environmental microbiology2003
Extremely acidophilic protists from acid mine drainage host Rickettsiales-lineage endosymbionts that have intervening sequences in their 16S rRNA genes.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 96 citations in OpenAlex.
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- Comprehensive analysis of insertion sequences within rRNA genes of CPR bacteria and biochemical characterization of a homing endonuclease encoded by these sequences.Journal of bacteriology · 2024Article
- "Candidatus Intestinibacterium parameciiphilum"-member of the "Candidatus Paracaedibacteraceae" family (Alphaproteobacteria, Holosporales) inhabiting the ciliated protist Paramecium.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
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- Isolation and Genome Analysis of an Amoeba-Associated BacteriumFrontiers in microbiology · 2022Article
- Biosynthetic capacity, metabolic variety and unusual biology in the CPR and DPANN radiations.Nature reviews. Microbiology · 2018Review
- Synchrony of Eukaryotic and Prokaryotic Planktonic Communities in Three Seasonally Sampled Austrian Lakes.Frontiers in microbiology · 2018Article
- Dynamics of Bacterial Communities Mediating the Treatment of an As-Rich Acid Mine Drainage in a Field Pilot.Frontiers in microbiology · 2018Article
- Eukaryotic molecular diversity at different steps of the wastewater treatment plant process reveals more phylogenetic novel lineages.World journal of microbiology & biotechnology · 2017Article
- Bacterial Community Structures in Freshwater Polar Environments of Svalbard.Microbes and environments · 2016Article
- Disentangling the Taxonomy of Rickettsiales and Description of Two Novel Symbionts ("Candidatus Bealeia paramacronuclearis" and "Candidatus Fokinia cryptica") Sharing the Cytoplasm of the Ciliate Protist Paramecium biaurelia.Applied and environmental microbiology · 2016Article
- Single sample resolution of rare microbial dark matter in a marine invertebrate metagenome.Scientific reports · 2016Article
- Communities that thrive in extreme conditions captured from a freshwater lake.Biology letters · 2016Article
- "Candidatus Finniella" (Rickettsiales, Alphaproteobacteria), Novel Endosymbionts of Viridiraptorid Amoeboflagellates (Cercozoa, Rhizaria).Applied and environmental microbiology · 2016Article
- The Intervening Sequence of Coxiella burnetii: Characterization and Evolution.Frontiers in cellular and infection microbiology · 2016Article
- Novel and Unexpected Microbial Diversity in Acid Mine Drainage in Svalbard (78° N), Revealed by Culture-Independent Approaches.Microorganisms · 2015Article
- Article
- Phylogeny of intestinal ciliates, including Charonina ventriculi, and comparison of microscopy and 18S rRNA gene pyrosequencing for rumen ciliate community structure analysis.Applied and environmental microbiology · 2015Article
- Simultaneous specific in planta visualization of root-colonizing fungi using fluorescence in situ hybridization (FISH).Mycorrhiza · 2014Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During a molecular phylogenetic survey of extremely acidic (pH < 1), metal-rich acid mine drainage habitats in the Richmond Mine at Iron Mountain, Calif., we detected 16S rRNA gene sequences of a novel bacterial group belonging to the order Rickettsiales in the Alphaproteobacteria. The closest known relatives of this group (92% 16S rRNA gene sequence identity) are endosymbionts of the protist Acanthamoeba. Oligonucleotide 16S rRNA probes were designed and used to observe members of this group within acidophilic protists. To improve visualization of eukaryotic populations in the acid mine drainage samples, broad-specificity probes for eukaryotes were redesigned and combined to highlight this component of the acid mine drainage community. Approximately 4% of protists in the acid mine drainage samples contained endosymbionts. Measurements of internal pH of the protists showed that their cytosol is close to neutral, indicating that the endosymbionts may be neutrophilic. The endosymbionts had a conserved 273-nucleotide intervening sequence (IVS) in variable region V1 of their 16S rRNA genes. The IVS does not match any sequence in current databases, but the predicted secondary structure forms well-defined stem loops. IVSs are uncommon in rRNA genes and appear to be confined to bacteria living in close association with eukaryotes. Based on the phylogenetic novelty of the endosymbiont sequences and initial culture-independent characterization, we propose the name "Candidatus Captivus acidiprotistae." To our knowledge, this is the first report of an endosymbiotic relationship in an extremely acidic habitat.
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