ArticleApplied and environmental microbiology2003
Assessment of methods for detection of infectious Cryptosporidium oocysts and Giardia cysts in reclaimed effluents.
Article in Applied and environmental microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Burden and Epidemiology of Human IntestinalTropical medicine and infectious disease · 2022Review
- Detection ofMicroorganisms · 2022Review
- Wastewater-based epidemiology-surveillance and early detection of waterborne pathogens with a focus on SARS-CoV-2, Cryptosporidium and Giardia.Parasitology research · 2021Review
- Biofilm Sampling for Detection ofApplied and environmental microbiology · 2020Article
- Copro-microscopical and immunological diagnosis of cryptosporidiosis in Egyptian buffalo-calves with special reference to their cytokine profiles.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2020Article
- Label-Free Capacitive Biosensor for Detection ofSensors (Basel, Switzerland) · 2019Article
- Protozoan Parasites in Drinking Water: A System Approach for Improved Water, Sanitation and Hygiene in Developing Countries.International journal of environmental research and public health · 2018Review
- Quantification of Protozoa and Viruses from Small Water Volumes.International journal of environmental research and public health · 2015Article
- Article
- Comparison of assays for sensitive and reproducible detection of cell culture-infectious Cryptosporidium parvum and Cryptosporidium hominis in drinking water.Applied and environmental microbiology · 2012Article
- Cryptosporidium and Giardia detection in environmental waters of southwest coastal areas of Thailand.Parasitology research · 2010Article
- Molecular characterization of sewage-borne pathogens and detection of sewage markers in an urban stream in Caracas, Venezuela.Applied and environmental microbiology · 2010Article
- Improved risk analysis by dual direct detection of total and infectious Cryptosporidium oocysts on cell culture in combination with immunofluorescence assay.Applied and environmental microbiology · 2010Article
- Detection of Cryptosporidium oocysts in water: effect of the number of samples and analytic replicates on test results.Applied and environmental microbiology · 2006Article
- Detection of infectious Cryptosporidium oocysts by cell culture immunofluorescence assay: applicability to environmental samples.Applied and environmental microbiology · 2005Article
- Validity of the indicator organism paradigm for pathogen reduction in reclaimed water and public health protection.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluates the occurrence of Cryptosporidium oocysts and Giardia cysts in reclaimed effluents if method 1623 with the Envirochek capsule filters (standard and high-volume [HV] filters) and a modified version of the Information Collection Rule method (ICR) with the polypropylene yarn-wound cartridge filter are used. The recovery efficiency of the analytical methods was evaluated with samples of reagent, tap, and reclaimed water by using flow cytometer-sorted spike suspensions. (Oo)cyst recovery efficiency determined filter performance and method reproducibility in the water matrix tested. Method 1623 with the Envirochek HV capsule filter generated significantly higher recovery rates than did the standard Envirochek filter and the modified ICR method. Notwithstanding, large variations in recovery rates (>80%) occurred with samples of reclaimed water, and none of the water quality parameters analyzed in the reclaimed effluents could explain such variability. The highest concentrations of indigenous oocysts were detected by method 1623 with the HV filter, which provided a sufficient number of oocysts for further confirmation of infectious potential. Confirmation of species and potential infectivity for all positive protozoan samples was made by using a nested PCR restriction fragment polymorphism assay and the focus detection method most-probable-number assay, respectively. The methodology and results described in the present investigation provide useful information for the establishment of pathogen numeric standards for reclaimed effluents used for unrestricted irrigation.
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