ArticlePlant molecular biology2003
Rare instances of Cre-mediated deletion product maintained in transgenic wheat.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Clean gene technology to develop selectable marker-free pod borer-resistant transgenic pigeon pea events involving the constitutive expression of Cry1Ac.Applied microbiology and biotechnology · 2022Article
- Level of tissue differentiation influences the activation of a heat-inducible flower-specific system for genetic containment in poplar (Populus tremula L.).Plant cell reports · 2016Article
- Article
- Split-Cre complementation restores combination activity on transgene excision in hair roots of transgenic tobacco.PloS one · 2014Article
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Article
- Distinct cell-autonomous functions of RETINOBLASTOMA-RELATED in Arabidopsis stem cells revealed by the Brother of Brainbow clonal analysis system.The Plant cell · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Article
- ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome.Transgenic research · 2009Article
- Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.Plant cell reports · 2008Article
- Quantification of Cre-mediated recombination by a novel strategy reveals a stable extra-chromosomal deletion-circle in mice.BMC biotechnology · 2008Article
- Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system.Plant physiology · 2007Article
- Self-excision of the antibiotic resistance gene nptII using a heat inducible Cre-loxP system from transgenic potato.Plant molecular biology · 2006Article
- Size does matter: cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites.Plant molecular biology · 2005Article
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Authors and funding
2 authors.
Funding
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Abstract
Previously, we described a Cre-lox based strategy to convert a complex multi-copy integration pattern to a single-copy transgene (Srivastava et al., 1999). When a lox-containing transgenic line of wheat was crossed with a cre-expressing line, extra copies of the transgene were deleted by site-specific recombination. This process included the removal of a lox-flanked selection marker gene, bar. Three out of six F1 plants were chimeric for the resolved and the complex loci because both completely resolved and incompletely resolved patterns were found in the F2 population. From one F1 plant, 4 out of 20 F2 progeny showed not only incomplete resolution of the complex integration pattern, but also the presence of a circular loxP-bar-nos3' fragment, which we refer to as the bar circle. This bar circle was detected in subsequent generations, and was associated with the presence of both the lox transgene and the cre locus. We hypothesize that the cre gene in these bar circle plants must have undergone a genetic or epigenetic change that altered the spatial and/or temporal pattern of cre expression. Late expression might excise the DNA incompletely, and late in development. What is surprising is that the DNA is not degraded, but remains in the cells as an extra-chromosomal circular molecule.
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