ArticleEuropean biophysics journal : EBJ2003
Fluorescence imaging with two-photon evanescent wave excitation.
Article in European biophysics journal : EBJ, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Three-dimensional plasmonic nanopores for DNA-PAINT and dual-material Au/Si architectures.Journal of nanobiotechnology · 2026Article
- Optometry for a short-sighted microscope.Biophysical journal · 2021Article
- Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy.Biophysical journal · 2019Review
- Eliminating unwanted far-field excitation in objective-type TIRF. Part I. identifying sources of nonevanescent excitation light.Biophysical journal · 2014Article
- Total internal reflection with fluorescence correlation spectroscopy: Applications to substrate-supported planar membranes.Journal of structural biology · 2009Article
- Detecting fluorescent protein expression and co-localisation on single secretory vesicles with linear spectral unmixing.European biophysics journal : EBJ · 2006Article
- Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis.The Journal of physiology · 2005Article
- A deeper look into single-secretory vesicle dynamics.Biophysical journal · 2004Review
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Authors and funding
3 authors.
Funding
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Abstract
We demonstrate broad-field, non-scanning, two-photon excitation fluorescence (2PEF) close to a glass/cell interface by total internal reflection of a femtosecond-pulsed infrared laser beam. We exploit the quadratic intensity dependence of 2PEF to provide non-linear evanescent wave (EW) excitation in a well-defined sample volume and to eliminate scattered background excitation. A simple model is shown to describe the resulting 2PEF intensity and to predict the effective excitation volume in terms of easily measurable beam, objective and interface properties. We demonstrate non-linear evanescent wave excitation at 860 nm of acridine orange-labelled secretory granules in live chromaffin cells, and excitation at 900 nm of TRITC-phalloidin-actin/GPI-GFP double-labelled fibroblasts. The confined excitation volume and the possibility of simultaneous multi-colour excitation of several fluorophores make EW 2PEF particularly advantageous for quantitative microscopy, imaging biochemistry inside live cells, or biosensing and screening applications in miniature high-density multi-well plates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.