ArticleProceedings of the National Academy of Sciences of the United States of America2003
Maize-targeted mutagenesis: A knockout resource for maize.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- A comprehensive overview of yeast libraries and their role in advancing cell biology.The FEBS journal · 2026Review
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Beautiful and delicious mutants: The origins, fates, and benefits of molecular sequence variation in plant evolution and breeding.Plant physiology · 2025Review
- The role of mobile DNA elements in the dynamics of plant genome plasticity.Journal of experimental botany · 2025Review
- Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Breeding for improved digestibility and processing of lignocellulosic biomass inFrontiers in plant science · 2024Review
- A forward genetics approach integrating genome-wide association study and expression quantitative trait locus mapping to dissect leaf development in maize (Zea mays).The Plant journal : for cell and molecular biology · 2021Article
- CRISPR screens in plants: approaches, guidelines, and future prospects.The Plant cell · 2021Review
- Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021Review
- Article
- High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize.The Plant cell · 2020Article
- Article
- A Sequence-IndexedPlant physiology · 2019Article
- Technological advances in maize breeding: past, present and future.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber.Journal of experimental botany · 2019Article
- An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.The Plant cell · 2016Article
- Expression of Phenylalanine Ammonia Lyase Genes in Maize Lines Differing in Susceptibility to Meloidogyne incognita.Journal of nematology · 2014Article
- Phenotype to genotype using forward-genetic Mu-seq for identification and functional classification of maize mutants.Frontiers in plant science · 2014Article
- Maize transformation technology development for commercial event generation.Frontiers in plant science · 2014Review
- Identification of the maize gravitropism gene lazy plant1 by a transposon-tagging genome resequencing strategy.PloS one · 2014Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe an efficient system for site-selected transposon mutagenesis in maize. A total of 43,776 F1 plants were generated by using Robertson's Mutator (Mu) pollen parents and self-pollinated to establish a library of transposon-mutagenized seed. The frequency of new seed mutants was between 10-4 and 10-5 per F1 plant. As a service to the maize community, maize-targeted mutagenesis selects insertions in genes of interest from this library by using the PCR. Pedigree, knockout, sequence, phenotype, and other information is stored in a powerful interactive database (maize-targeted mutagenesis database) that enables analysis of the entire population and the handling of knockout requests. By inhibiting Mu activity in most F1 plants, we sought to reduce somatic insertions that may cause false positives selected from pooled tissue. By monitoring the remaining Mu activity in the F2, however, we demonstrate that seed phenotypes depend on it, and false positives occur in lines that appear to lack it. We conclude that more than half of all mutations arising in this population are suppressed on losing Mu activity. These results have implications for epigenetic models of inbreeding and for functional genomics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.