ArticleNucleic acids research2003
Retrovirus silencer blocking by the cHS4 insulator is CTCF independent.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 62 citations in OpenAlex.
- Insulated piggyBac and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells.Biology open · 2025Article
- Investigating the Barrier Activity of Novel, Human Enhancer-Blocking Chromatin Insulators for Hematopoietic Stem Cell Gene Therapy.Human gene therapy · 2021Article
- Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE).BMC biotechnology · 2019Article
- Gene Therapy Tools for Brain Diseases.Frontiers in pharmacology · 2019Review
- Cellular Antisilencing Elements Support Transgene Expression from Herpes Simplex Virus Vectors in the Absence of Immediate Early Gene Expression.Journal of virology · 2018Article
- Insulators to Improve the Safety of Retroviral Vectors for HIV Gene Therapy.Biomedicines · 2016Article
- Chicken β-globin insulators fail to shield the nkx2.5 promoter from integration site effects in zebrafish.Molecular genetics and genomics : MGG · 2013Article
- Kinetics and epigenetics of retroviral silencing in mouse embryonic stem cells defined by deletion of the D4Z4 element.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Conserved boundary elements from the Hox complex of mosquito, Anopheles gambiae.Nucleic acids research · 2013Article
- DNA fragments binding CTCF in vitro and in vivo are capable of blocking enhancer activity.BMC research notes · 2012Article
- Developing genetically engineered mouse models to study tumor suppression.Current protocols in mouse biology · 2012Article
- CTF/NF1 transcription factors act as potent genetic insulators for integrating gene transfer vectors.Gene therapy · 2012Article
- Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity.PLoS genetics · 2011Article
- The use of chromatin insulators to improve the expression and safety of integrating gene transfer vectors.Human gene therapy · 2011Review
- Strategies to insulate lentiviral vector-expressed transgenes.Methods in molecular biology (Clifton, N.J.) · 2010Article
- VEZF1 elements mediate protection from DNA methylation.PLoS genetics · 2010Article
- Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells.Nucleic acids research · 2009Article
- Article
- Vertebrate Protein CTCF and its Multiple Roles in a Large-Scale Regulation of Genome Activity.Current genomics · 2009Article
- Diverse transcription influences can be insulated by the Drosophila SF1 chromatin boundary.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silencing of retrovirus vectors poses a significant obstacle to genetic manipulation of stem cells and their use in gene therapy. We describe a mammalian silencer blocking assay using insulator elements positioned between retrovirus silencer elements and an LCRbeta-globin reporter transgene. In transgenic mice, we show that retrovirus silencers are blocked by the cHS4 insulator. Silencer blocking is independent of the CTCF binding site and is most effective when flanking the internal reporter transgene. These data distinguish silencer blocking activity by cHS4 from its enhancer blocking activity. Retrovirus vectors can be created at high titer with one but not two internal dimer cHS4 cores. cHS4 in the LTRs has no effect on expression in transduced F9 cells, suggesting that position effect blocking is not sufficient to escape silencing. The Drosophila insulators gypsy and Scs fail to block silencing in transgenic mice, but gypsy stimulates vector expression 2-fold when located in the LTRs of an infectious retrovirus. The silencer blocking assay complements existing insulator assays in mammalian cells, provides new insight into mechanisms of insulation and is a valuable tool to identify additional silencer blocking insulators that cooperate with cHS4 to improve stem cell retrovirus vector design.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.