ArticleNucleic acids research2003
Rad52 and Ku bind to different DNA structures produced early in double-strand break repair.
Article in Nucleic acids research, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 56 citations in OpenAlex.
- The human RAD52 complex undergoes phase separation and facilitates bundling and end-to-end tethering of RAD51 presynaptic filaments.Nucleic acids research · 2026Article
- Monomers and short oligomers of human RAD52 promote single-strand annealing.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The hydrophilic extract from a new tomato genotype (named DHO) kills cancer cell lines through the modulation of the DNA damage response induced by Campthotecin treatment.Frontiers in oncology · 2023Article
- Analysis of nuclear maturation, DNA damage and repair gene expression of bovine oocyte and cumulus cells submitted to ionizing radiation.Animal reproduction · 2023Article
- Yeast ATM and ATR kinases use different mechanisms to spread histone H2A phosphorylation around a DNA double-strand break.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RAD52 is required for RNA-templated recombination repair in post-mitotic neurons.The Journal of biological chemistry · 2018Article
- Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells.The EMBO journal · 2017Article
- Microhomology-mediated end joining is activated in irradiated human cells due to phosphorylation-dependent formation of the XRCC1 repair complex.Nucleic acids research · 2017Article
- Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination.Nature communications · 2017Article
- Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks.Nucleic acids research · 2016Article
- Article
- DNA damage response and repair: insights into strategies for radiation sensitization of gliomas.Future oncology (London, England) · 2011Review
- RecO-mediated DNA homology search and annealing is facilitated by SsbA.Nucleic acids research · 2010Article
- Molecular recognition of DNA-protein complexes: a straightforward method combining scanning force and fluorescence microscopy.Ultramicroscopy · 2010Article
- Human replication protein A-Rad52-single-stranded DNA complex: stoichiometry and evidence for strand transfer regulation by phosphorylation.Biochemistry · 2009Article
- Hierarchy of nonhomologous end-joining, single-strand annealing and gene conversion at site-directed DNA double-strand breaks.Nucleic acids research · 2008Article
- Alternative-NHEJ is a mechanistically distinct pathway of mammalian chromosome break repair.PLoS genetics · 2008Article
- Non-homologous end-joining, a sticky affair.Oncogene · 2007Review
- In my end is my beginning: control of end resection and DSBR pathway 'choice' by cyclin-dependent kinases.Oncogene · 2005Review
- Enhancing gene targeting efficiency in higher plants: rice is on the move.Transgenic research · 2005Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA double-strand breaks are repaired by one of two main pathways, non-homologous end joining or homologous recombination. A competition for binding to DNA ends by Ku and Rad52, proteins required for non-homologous end joining and homologous recombination, respectively, has been proposed to determine the choice of repair pathway. In order to test this idea directly, we compared Ku and human Rad52 binding to different DNA substrates. How ever, we found no evidence that these proteins would compete for binding to the same broken DNA ends. Ku bound preferentially to DNA with free ends. Under the same conditions, Rad52 did not bind preferentially to DNA ends. Using a series of defined substrates we showed that it is single-stranded DNA and not DNA ends that were preferentially bound by Rad52. In addition, Rad52 aggregated DNA, bringing different single-stranded DNAs in close proximity. This activity was independent of the presence of DNA ends and of the ability of the single-stranded sequences to form extensive base pairs. Based on these DNA binding characteristics it is unlikely that Rad52 and Ku compete as 'gatekeepers' of different DNA double-strand break repair pathways. Rather, they interact with different DNA substrates produced early in DNA double-strand break repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.