ArticleThe Plant cell2003
Phosphorylation of the potyvirus capsid protein by protein kinase CK2 and its relevance for virus infection.
Article in The Plant cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 140 citations in OpenAlex.
- Chicken infectious anemia virus exploits host CK2α as an essential factor for its replication via VP2 Ser182/Asp183-mediated interaction.Journal of virology · 2026Article
- Generation of Zucchini Tigre Mosaic Virus Mild Strains for Application in Cross-Protection.Viruses · 2026Article
- Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal region.Journal of virology · 2025Article
- Oligomeric SWEET1g of Solanum tuberosum confers resistance to potato virus Y and Potato virus X.The Plant journal : for cell and molecular biology · 2025Article
- Review
- The C4 photosynthesis bifunctional enzymes, PDRPs, of maize are co-opted to cytoplasmic viral replication complexes to promote infection of a prevalent potyvirus sugarcane mosaic virus.Plant biotechnology journal · 2024Article
- Bioinformatics Insights on Viral Gene Expression Transactivation: From HIV-1 to SARS-CoV-2.International journal of molecular sciences · 2024Article
- From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus Y.Communications chemistry · 2024Article
- Five questions on the cell-to-cell movement ofBBA advances · 2024Article
- 6-(Tetrazol-5-yl)-7-aminoazolo[1,5-Molecules (Basel, Switzerland) · 2022Article
- Article
- Interplay of HCPro and CP in the Regulation of Potato Virus A RNA Expression and Encapsidation.Viruses · 2022Article
- Occurrence and Genetic Characterization of Grapevine Pinot Gris Virus in Russia.Plants (Basel, Switzerland) · 2022Article
- StREM1.3 REMORIN Protein Plays an Agonistic Role in Potyvirus Cell-to-Cell Movement inViruses · 2022Article
- Phosphorylation of plant virus proteins: Analysis methods and biological functions.Frontiers in microbiology · 2022Review
- The conserved aromatic residue WMolecular plant pathology · 2021Article
- Functional Characterization of Pepper Vein Banding Virus-Encoded Proteins and Their Interactions: Implications inViruses · 2020Review
- Review
- Article
- Dynamics of Protein Accumulation from the 3' End of Viral RNA Are Different from Those in the Rest of the Genome in Potato Virus A Infection.Journal of virology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We reported previously that the capsid protein (CP) of Potato virus A (PVA) is phosphorylated both in virus-infected plants and in vitro. In this study, an enzyme that phosphorylates PVA CP was identified as the protein kinase CK2. The alpha-catalytic subunit of CK2 (CK2alpha) was purified from tobacco and characterized using in-gel kinase assays and liquid chromatography-tandem mass spectrometry. The tobacco CK2alpha gene was cloned and expressed in bacterial cells. Specific antibodies were raised against the recombinant enzyme and used to demonstrate the colocalization of PVA CP and CK2alpha in infected tobacco protoplasts. A major site of CK2 phosphorylation in PVA CP was identified by a combination of mass spectrometric analysis, radioactive phosphopeptide sequencing, and mutagenesis as Thr-242 within a CK2 consensus sequence. Amino acid substitutions that affect the CK2 consensus sequence in CP were introduced into a full-length infectious cDNA clone of PVA tagged with green fluorescent protein. Analysis of the mutant viruses showed that they were defective in cell-to-cell and long-distance movement. Using in vitro assays, we demonstrated that CK2 phosphorylation inhibited the binding of PVA CP to RNA, suggesting a molecular mechanism of CK2 action. These results suggest that the phosphorylation of PVA CP by CK2 plays an important regulatory role in virus infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.