ArticleJournal of muscle research and cell motility2002
Mitochondrial membrane dynamics are altered in cluA- mutants of Dictyostelium.
Article in Journal of muscle research and cell motility, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- No Chance to Survive:Antibiotics (Basel, Switzerland) · 2023Article
- Simple to Complex: The Role of Actin and Microtubules in Mitochondrial Dynamics in Amoeba, Yeast, and Mammalian Cells.International journal of molecular sciences · 2022Review
- Clueless/CLUH regulates mitochondrial fission by promoting recruitment of Drp1 to mitochondria.Nature communications · 2022Article
- The interactome of CLUH reveals its association to SPAG5 and its co-translational proximity to mitochondrial proteins.BMC biology · 2022Article
- FMT, a protein that affects mitochondrial distribution, interacts with translation-related proteins in Arabidopsis thaliana.Plant cell reports · 2021Article
- CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy.The EMBO journal · 2020Article
- The influence of mitochondrial dynamics on mitochondrial genome stability.Current genetics · 2018Article
- CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.The Journal of cell biology · 2017Article
- Drosophila clueless is involved in Parkin-dependent mitophagy by promoting VCP-mediated Marf degradation.Human molecular genetics · 2016Article
- Microtubules Are Essential for Mitochondrial Dynamics-Fission, Fusion, and Motility-in Dictyostelium discoideum.Frontiers in cell and developmental biology · 2016Article
- CLUH regulates mitochondrial biogenesis by binding mRNAs of nuclear-encoded mitochondrial proteins.The Journal of cell biology · 2014Article
- FRIENDLY regulates mitochondrial distribution, fusion, and quality control in Arabidopsis.Plant physiology · 2014Article
- The mitochondrial Ras-related GTPase Miro: views from inside and outside the metazoan kingdom.Frontiers in plant science · 2014Review
- Farnesyl diphosphate synthase, the target for nitrogen-containing bisphosphonate drugs, is a peroxisomal enzyme in the model system Dictyostelium discoideum.The Biochemical journal · 2012Article
- Mitochondrial fission and fusion in Dictyostelium discoideum: a search for proteins involved in membrane dynamics.BMC research notes · 2012Article
- CLUMPED CHLOROPLASTS 1 is required for plastid separation in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- A Dictyostelium SH2 adaptor protein required for correct DIF-1 signaling and pattern formation.Developmental biology · 2011Article
- Mitochondrial dynamics.The New phytologist · 2003Review
- Two Dictyostelium orthologs of the prokaryotic cell division protein FtsZ localize to mitochondria and are required for the maintenance of normal mitochondrial morphology.Eukaryotic cell · 2003Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In cluA- mutants of Dictyostelium, mitochondria are clustered near the cell center rather than being dispersed throughout the cytoplasm. We have examined two possible mechanisms that could account for this phenotype. First, we sought evidence that the cytoskeleton or a presumptive mitochondrion-cytoskeleton linkage was altered in mutant cells. We found that cytoskeletal structures in cluA- cells appeared normal by immunostaining, and that the distribution of peroxisomes in mutant cells was indistinguishable from that in wild type cells. Treatment of wild type cells with drugs that disrupted microtubules or actin filaments did not mimic the cluA- phenotype. Thus, cytoskeletal defects seemed unlikely to account for the mitochondrial clustering in cluA- cells. Observation of the movement of GFP-tagged mitochondria in wild type cells suggested that mitochondria are transported along microtubules, as in mammalian cells, rather than along actin filaments, as in budding yeast. Therefore, the similar phenotypes of cluA- Dictyostelium cells and clu1delta yeast cells argued against CluA/Clu1p acting as a mitochondrion-cytoskeleton linker. We next examined the ultrastructure of mitochondria in freeze-substituted, thin-sectioned cells. We found that the clustered mitochondria in cluA- cells are interconnected. Often, adjacent mitochondria are linked by narrow membranous strands, although sometimes the mitochondria are partially merged. The presence of narrow constrictions at presumptive division sites argues that the constriction step of division proceeds normally. Our data suggest that cluA- cells may be blocked at a very late step in fission of the outer mitochondrial membrane.
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