Evidence map›Paper›PMID 12949223›Full record

Trial reportThe Journal of physiology2003

Blunted sympathetic vasoconstriction in contracting skeletal muscle of healthy humans: is nitric oxide obligatory?

Frank A Dinenno, Michael J Joyner

Open access · greenAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.

0numbers the graph read from it
0cells of the map it votes in
81citing papers in PubMed
6.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

81 citing papers in PubMed, 168 citations in OpenAlex.

  1. Trial
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  3. The role of vascular endothelium in nitroglycerin-mediated vasodilation.British journal of clinical pharmacology · 2019
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  8. Vasoconstrictor responsiveness during hyperbaric hyperoxia in contracting human muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2013
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  12. Alpha-adrenergic control of blood flow during exercise: effect of sex and menstrual phase.Journal of applied physiology (Bethesda, Md. : 1985) · 2010
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  13. Intradermal administration of ATP does not mitigate tyramine-stimulated vasoconstriction in human skin.American journal of physiology. Regulatory, integrative and comparative physiology · 2010
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21 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Frank A DinennoDepartment of Anesthesiology and General Clinical Research Center, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Michael J Joyner
Mayo Clinic · US

Funding

ZESTRIL VS. VERAPAMIL IN THE TREATMENT OF HYPERTENSIONM01RR000585 · NCRR · MAYO CLINIC ROCHESTER · PI RIZZA, ROBERT A. · 1985 to 2006
$30.4M
SYMPATHETIC REGULATION OF VENOUS FUNCTION IN ORTHOSTATIC INTOLERANCE AND AGINGP50NS032352 · NINDS · MAYO CLINIC ROCHESTER · PI LOW, PHILLIP A · 1996 to 2009
$11.4M
PERFUSION OF ACTIVE MUSCLES--METABOLITES AND NERVESR01HL046493 · NHLBI · MAYO CLINIC ROCHESTER · PI JOYNER, MICHAEL J · 1997 to 2011
$3.7M
AGING AND ALPHA-ADRENERGIC CONTROL OF MUSCLE BLOOD FLOWF32AG005912 · NIA · MAYO CLINIC COLL OF MEDICINE, ROCHESTER · PI DINENNO, FRANK A · 2000 to 2002
$107k
ORTHOSTATIC INTOLERANCE IN AUTONOMIC NEUROPATHIES &POSTURAL TACHYCARDIA SYNDROMEP01NS032352 · NINDS · MAYO CLINIC COLL OF MEDICINE, ROCHESTER · PI BENARROCH, EDUARDO E · 1995 to 1995
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ELECTRODE ARRAYS BASED ON THIN FILM TECHNOLOGYN01NS032352 · NINDS · STANFORD UNIVERSITY · PI WHITE, ROBERT L · 1985 to 1985
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PERFUSION OF ACTIVE MUSCLES: METABOLITES AND NERVESR29HL046493 · NHLBI · MAYO CLINIC COLL OF MEDICINE, ROCHESTER · PI JOYNER, MICHAEL J · 1992 to 1996
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NCRR NIH HHS M01 RR000585NCRR NIH HHS RR-00585NHLBI NIH HHS HL-46493NHLBI NIH HHS R01 HL046493NIA NIH HHS AG-05912NIA NIH HHS F32 AG005912NINDS NIH HHS N01 NS032352NINDS NIH HHS NS-32352
6 · The paper itself

Abstract

We tested the hypothesis that nitric oxide (NO) is responsible for blunting sympathetic alpha-adrenergic vasoconstriction in the active muscles of humans (functional sympatholysis). We measured forearm blood flow (Doppler ultrasound) and calculated the reductions in forearm vascular conductance (FVC) in response to alpha-adrenergic receptor stimulation during rhythmic handgrip exercise and during a control non-exercise vasodilator condition (intra-arterial adenosine), before and after local NO synthase (NOS) inhibition in healthy men. The forearm vasoconstrictor responses to endogenous noradrenaline release (intra-arterial tyramine) were significantly blunted during moderate exercise compared with adenosine, and these vasoconstrictor responses were not restored by NOS inhibition with NG-monomethyl-L-arginine (L-NMMA; n = 6) or NG-nitro-L-arginine methyl ester (L-NAME; n = 8). Similarly, L-NAME did not restore the vasoconstrictor responses to tyramine in contracting muscle during heavy rhythmic handgrip exercise (n = 4). In four additional subjects, we also found that the vasoconstrictor responses evoked by tyramine during exercise or adenosine were repeatable in the absence of NOS inhibition (i.e. time control). Finally, in five subjects the forearm vasoconstrictor responses to direct alpha 1-adrenergic (phenylephrine) and alpha 2-adrenergic (clonidine) receptor stimulation were blunted during moderate exercise compared with adenosine; these responses were also unaffected by L-NAME. Taken together, our results demonstrate that NO is not obligatory for functional sympatholysis in contracting skeletal muscles of healthy men.

Indexed as

AdenosineAdultEnzyme InhibitorsForearmHand StrengthHumansMaleMuscle ContractionMuscle, SkeletalNitric Oxideomega-N-MethylarginineRegional Blood FlowSympathetic Nervous SystemTyramineVasoconstrictionVasodilator AgentsAdenosineEnzyme InhibitorsNitric Oxideomega-N-MethylarginineTyramineVasodilator Agents

Identifiers

PMID12949223
PMCPMC2343482
OpenAlexW2030958375

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.