ArticleJournal of bacteriology2003
Epoxyalkane: coenzyme M transferase in the ethene and vinyl chloride biodegradation pathways of mycobacterium strain JS60.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 103 citations in OpenAlex.
- Control of alluvial aquifer architecture on reductive and oxidative dechlorination of chloroethenes.Environmental science and pollution research international · 2026Article
- Phylogenetic and Functional Diversity of Soluble Di-Iron Monooxygenases.Environmental microbiology · 2025Review
- Ethylene and epoxyethane metabolism in methanotrophic bacteria: comparative genomics and physiological studies usingMicrobial genomics · 2024Article
- Development of a whole-cell biosensor for ethylene oxide and ethylene.Microbial biotechnology · 2024Article
- Contrasting regulatory effects of organic acids on aerobic vinyl chloride biodegradation in etheneotrophs.Applied microbiology and biotechnology · 2022Article
- Microbiome Composition and Dynamics of a Reductive/Oxidative Bioelectrochemical System for Perchloroethylene Removal: Effect of the Feeding Composition.Frontiers in microbiology · 2022Article
- Synthetic Biology Approaches to Hydrocarbon Biosensors: A Review.Frontiers in bioengineering and biotechnology · 2021Review
- Comparative Genomic Study of Vinyl Chloride Cluster and Description of Novel Species,Frontiers in microbiology · 2021Article
- Effect of biodegradation on haloacetic acid formation potentials of anthropogenic compounds during chlorination.Environmental science and pollution research international · 2020Article
- Genetic and Physiological Characteristics of a Novel Marine Propylene-Assimilating Halieaceae Bacterium Isolated from Seawater and the Diversity of Its Alkene and Epoxide Metabolism Genes.Microbes and environments · 2019Article
- Heterologous Expression of Mycobacterium Alkene Monooxygenases in Gram-Positive and Gram-Negative Bacterial Hosts.Applied and environmental microbiology · 2018Article
- Ethylene and 1-Aminocyclopropane-1-carboxylate (ACC) in Plant-Bacterial Interactions.Frontiers in plant science · 2018Review
- Biodegradation of Volatile Organic Compounds and Their Effects on Biodegradability under Co-Existing Conditions.Microbes and environments · 2017Review
- Microbial degradation of chloroethenes: a review.Environmental science and pollution research international · 2017Review
- Temporal abundance and activity trends of vinyl chloride (VC)-degrading bacteria in a dilute VC plume at Naval Air Station Oceana.Environmental science and pollution research international · 2017Article
- Epoxyalkane:Coenzyme M Transferase Gene Diversity and Distribution in Groundwater Samples from Chlorinated-Ethene-Contaminated Sites.Applied and environmental microbiology · 2016Article
- Metabolism of 2-methylpropene (isobutylene) by the aerobic bacterium Mycobacterium sp. strain ELW1.Applied and environmental microbiology · 2015Article
- SmoXYB1C1Z of Mycobacterium sp. strain NBB4: a soluble methane monooxygenase (sMMO)-like enzyme, active on C2 to C4 alkanes and alkenes.Applied and environmental microbiology · 2014Article
- Cytochrome P450 initiates degradation of cis-dichloroethene by Polaromonas sp. strain JS666.Applied and environmental microbiology · 2013Article
- Isolation of two novel marine ethylene-assimilating bacteria, Haliea species ETY-M and ETY-NAG, containing particulate methane monooxygenase-like genes.Microbes and environments · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycobacterium strains that grow on ethene and vinyl chloride (VC) are widely distributed in the environment and are potentially useful for biocatalysis and bioremediation. The catabolic pathway of alkene assimilation in mycobacteria is not well characterized. It is clear that the initial step is a monooxygenase-mediated epoxidation that produces epoxyethane from ethene and chlorooxirane from VC, but the enzymes involved in subsequent transformation of the epoxides have not been identified. We investigated epoxyethane metabolism in Mycobacterium strain JS60 and discovered a coenzyme M (CoM)-dependent enzyme activity in extracts from VC- and ethene-grown cells. PCR amplifications using primers targeted at epoxyalkane:CoM transferase (EaCoMT) genes yielded part of the JS60 EaCoMT gene, which was used to clone an 8.4-kb genomic DNA fragment. The complete EaCoMT gene (etnE) was recovered, along with genes (etnABCD) encoding a four-component monooxygenase and two genes possibly involved in acyl-CoA ester metabolism. Reverse transcription-PCR indicated that the etnE and etnA genes were cotranscribed and inducible by ethene and VC. Heterologous expression of the etnE gene in Mycobacterium smegmatis mc(2)155 using the pMV261 vector gave a recombinant strain capable of transforming epoxyethane, epoxypropane, and chlorooxirane. A metabolite identified by mass spectrometry as 2-hydroxyethyl-CoM was produced from epoxyethane. The results indicate that the EaCoMT and monooxygenase enzymes encoded by a single operon (etnEABCD) catalyze the initial reactions in both the VC and ethene assimilation pathways. CoM-mediated reactions appear to be more widespread in bacteria than was previously believed.
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