ArticleJournal of bacteriology2003
In vivo reconstitution of the FhuA transport protein of Escherichia coli K-12.
Article in Journal of bacteriology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Phage-antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance.Journal of biomedical science · 2026Article
- Structural constraints of pyocin S2 import through the ferripyoverdine receptor FpvAI.PNAS nexus · 2024Article
- Deciphering Bacteriophage T5 Host Recognition Mechanism and Infection Trigger.Journal of virology · 2023Article
- Outer Membrane Biogenesis.Annual review of microbiology · 2017Review
- Going Outside the TonB Box: Identification of Novel FepA-TonB InteractionsJournal of bacteriology · 2017Article
- Structural basis for hijacking siderophore receptors by antimicrobial lasso peptides.Nature chemical biology · 2014Article
- Spanin function requires subunit homodimerization through intermolecular disulfide bonds.Molecular microbiology · 2013Article
- TonB-dependent transporter FhuA in planar lipid bilayers: partial exit of its plug from the barrel.Biochemistry · 2012Article
- Activation of colicin M by the FkpA prolyl cis-trans isomerase/chaperone.The Journal of biological chemistry · 2011Article
- Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- FhuA (TonA), the career of a protein.Journal of bacteriology · 2009Review
- Structure and function of Pseudomonas aeruginosa protein PA1324 (21-170).Protein science : a publication of the Protein Society · 2009Article
- Periplasmic chaperone FkpA is essential for imported colicin M toxicity.Molecular microbiology · 2008Article
- Evidence of ball-and-chain transport of ferric enterobactin through FepA.The Journal of biological chemistry · 2007Article
- Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens.Acta crystallographica. Section F, Structural biology and crystallization communications · 2006Article
- Activities of the Serratia marcescens heme receptor HasR and isolated plug and beta-barrel domains: the beta-barrel forms a heme-specific channel.Journal of bacteriology · 2005Article
- The generalized transducing Salmonella bacteriophage ES18: complete genome sequence and DNA packaging strategy.Journal of bacteriology · 2005Article
- Defined inactive FecA derivatives mutated in functional domains of the outer membrane transport and signaling protein of Escherichia coli K-12.Journal of bacteriology · 2004Article
- Loop deletions indicate regions important for FhuA transport and receptor functions in Escherichia coli.Journal of bacteriology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The FhuA protein in the outer membrane of Escherichia coli actively transports ferrichrome and the antibiotics albomycin and rifamycin CGP 4832 and serves as a receptor for the phages T1, T5, and phi80 and for colicin M and microcin J25. The crystal structure reveals a beta-barrel with a globular domain, the cork, which closes the channel formed by the barrel. Genetic deletion of the cork resulted in a beta-barrel that displays no FhuA activity. A functional FhuA was obtained by cosynthesis of separately encoded cork and the beta-barrel domain, each endowed with a signal sequence, which showed that complementation occurs after secretion of the fragments across the cytoplasmic membrane. Inactive complete mutant FhuA and an FhuA fragment containing 357 N-proximal amino acid residues complemented the separately synthesized wild-type beta-barrel to form an active FhuA. Previous claims that the beta-barrel is functional as transporter and receptor resulted from complementation by inactive complete FhuA and the 357-residue fragment. No complementation was observed between the wild-type cork and complete but inactive FhuA carrying cork mutations that excluded the exchange of cork domains. The data indicate that active FhuA is reconstituted extracytoplasmically by insertion of separately synthesized cork or cork from complete FhuA into the beta-barrel, and they suggest that in wild-type FhuA the beta-barrel is formed prior to the insertion of the cork.
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