ArticleProceedings of the National Academy of Sciences of the United States of America2003
Active self-splicing group I introns in 23S rRNA genes of hyperthermophilic bacteria, derived from introns in eukaryotic organelles.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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25 citing papers in PubMed, 46 citations in OpenAlex.
- Group I introns in tRNA genes of Patescibacteria.mSystems · 2026Article
- Proposal ofISME communications · 2025Article
- Comprehensive analysis of insertion sequences within rRNA genes of CPR bacteria and biochemical characterization of a homing endonuclease encoded by these sequences.Journal of bacteriology · 2024Article
- Importance of pre-mRNA splicing and its study tools in plants.Advanced biotechnology · 2024Review
- Article
- Group I introns are widespread in archaea.Nucleic acids research · 2018Article
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Multiple self-splicing introns in the 16S rRNA genes of giant sulfur bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Cordyceps militaris (Hypocreales: Cordycipitaceae): transcriptional analysis and molecular characterization of cox1 and group I intron with putative LAGLIDADG endonuclease.World journal of microbiology & biotechnology · 2012Article
- Evolution of introns in the archaeal world.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The Agaricus bisporus cox1 gene: the longest mitochondrial gene and the largest reservoir of mitochondrial group i introns.PloS one · 2010Article
- On the chimeric nature, thermophilic origin, and phylogenetic placement of the Thermotogales.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Survey of group I and group II introns in 29 sequenced genomes of the Bacillus cereus group: insights into their spread and evolution.Nucleic acids research · 2008Article
- Cyanobacterial ribosomal RNA genes with multiple, endonuclease-encoding group I introns.BMC evolutionary biology · 2007Article
- The unusual 23S rRNA gene of Coxiella burnetii: two self-splicing group I introns flank a 34-base-pair exon, and one element lacks the canonical omegaG.Journal of bacteriology · 2007Article
- The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif.The EMBO journal · 2007Article
- Inteins, introns, and homing endonucleases: recent revelations about the life cycle of parasitic genetic elements.BMC evolutionary biology · 2006Article
- Evidence for existence of "mesotogas," members of the order Thermotogales adapted to low-temperature environments.Applied and environmental microbiology · 2006Article
- Article
Corrections and comments
- Erratum issued
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group I introns are common in the 23 rRNA genes of mitochondria and chloroplasts. Often, they encode "homing endonucleases," which target highly conserved gene sequences and drive interorganellar intron mobility, even across species and genus lines. Most bacterial 23S rRNA genes show these same endonuclease-sensitive target sequences. However, only two bacterial 23S rRNA genes are known to contain group I introns: that of Simkania negevensis [Everett, K. D., Kahane, S., Bush, R. M. & Friedman, M. G. (1999) J. Bacteriol. 181, 4734-4740], where the intron is not spliced and probably limits growth, and that of Coxiella burnetii [Seshadri, R., et al. (2003) Proc. Natl. Acad. Sci. USA 100, 5455-5460], where no direct evidence of splicing exists. Both bacteria are intracellular parasites and might have acquired introns from eukaryotic hosts. Here we provide direct evidence for splicing, and evolutionary evidence for mobility, of group I introns in the 23S rRNA genes of several free-living hyperthermophilic bacteria of the genus Thermotoga. These bacteria do not live closely with eukaryotes, but phylogenetic analyses suggest that their introns were also acquired from eukaryotic (probably algal) organelles. In vivo, their introns must be spliced at temperatures approaching 90 degrees C, making them the most thermostable natural ribozymes so far described. We demonstrate that at least some of these introns can also self-splice in vitro.
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