ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Differential roles of engrailed paralogs in determining sensory axon guidance and synaptic target recognition.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Functional Divergence of theInsects · 2023Article
- Developmental Biology in Central America, the northern region of South America and the Caribbean.The International journal of developmental biology · 2021Review
- Clinical Phenotypes Associated to Engrailed 2 Gene Alterations in a Series of Neuropediatric Patients.Frontiers in neuroanatomy · 2018Article
- Engrailed homeoproteins in visual system development.Cellular and molecular life sciences : CMLS · 2015Review
- Engrailed alters the specificity of synaptic connections of Drosophila auditory neurons with the giant fiber.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Auditory responses of engrailed and invected-expressing Johnston's Organ neurons in Drosophila melanogaster.PloS one · 2013Article
- Mechanosensilla in the adult abdomen of Drosophila: engrailed and slit help to corral the peripheral sensory axons into segmental bundles.Development (Cambridge, England) · 2010Article
- Transcriptional control of behavior: engrailed knock-out changes cockroach escape trajectories.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Synapse formation in developing neural circuits.Current topics in developmental biology · 2009Review
- Engrailed expression in subsets of adult Drosophila sensory neurons: an enhancer-trap study.Invertebrate neuroscience : IN · 2008Article
- Octopamine-mediated neuromodulation of insect senses.Neurochemical research · 2007Review
- The evolution of hexapod engrailed-family genes: evidence for conservation and concerted evolution.Proceedings. Biological sciences · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The transcription factor Engrailed (En) controls axon pathfinding and synaptic target choice in an identified neuron (6m) of the cockroach cercal sensory system. Knock-out of En using double-stranded RNA interference (dsRNAi) transforms 6m so that it resembles a neighboring neuron that normally does not express the en gene, has a different arbor anatomy, and makes different connections. Like many animals, the cockroach has two En paralogs, Pa-En1 and Pa-En2. In this study we tested the hypothesis that the paralogs have different effects on axon guidance and synaptic target recognition, using RNAi to knock out each one individually. Using dye injections into 6m and intracellular recordings from target interneurons, we obtained evidence that both Pa-En1 and Pa-En2 determine the axonal arborization, but only Pa-En1 controls synaptic connections. However, because immunocytochemical quantification of En protein in 6m after RNAi showed that Pa-En1 represents 65% of the total En activity and Pa-En2 only 35%, our results could be caused by dosage effects. We measured the effects of diluting the mixture of both dsRNAs on the amounts of En protein. From this dose-response curve, we calculated the appropriate dilutions of the dsRNA mixture that would titrate total En protein to levels equivalent to knock-out of either paralog. RNAi using these dilutions showed that Pa-En1 and Pa-En2 both contribute toward the control of axonal guidance and confirmed that Pa-En1 has the paralog-specific function of controlling synaptic target recognition.
Indexed as
Identifiers
12944515PMC6740593W2167024115What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.