ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Novel insights into the regulation of the timeless protein.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 69 citations in OpenAlex.
- Splicing of a core circadian clock gene regulates seasonal adaptations by a winter gating mechanism.Science advances · 2026Article
- Post-Translational Modifications in Animal Circadian Clocks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Splicing of a core clock gene regulates seasonal adaptations by a winter gating mechanism.bioRxiv : the preprint server for biology · 2025Article
- The ubiquitin-proteasome system in circadian regulation.Frontiers in neuroscience · 2025Review
- Evolution of casein kinase 1 and functional analysis of newFrontiers in physiology · 2022Article
- CK2 Inhibits TIMELESS Nuclear Export and Modulates CLOCK Transcriptional Activity to Regulate Circadian Rhythms.Current biology : CB · 2021Article
- NewFrontiers in physiology · 2019Article
- GSK-3 and CK2 Kinases Converge on Timeless to Regulate the Master Clock.Cell reports · 2016Article
- Drosophila TIM binds importin α1, and acts as an adapter to transport PER to the nucleus.PLoS genetics · 2015Article
- A role for timely nuclear translocation of clock repressor proteins in setting circadian clock speed.Experimental neurobiology · 2014Review
- Dual PDF signaling pathways reset clocks via TIMELESS and acutely excite target neurons to control circadian behavior.PLoS biology · 2014Article
- Speed control: cogs and gears that drive the circadian clock.Trends in neurosciences · 2012Review
- Post-translational regulation and nuclear entry of TIMELESS and PERIOD are affected in new timeless mutant.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- A key temporal delay in the circadian cycle of Drosophila is mediated by a nuclear localization signal in the timeless protein.Genetics · 2011Article
- Of switches and hourglasses: regulation of subcellular traffic in circadian clocks by phosphorylation.EMBO reports · 2010Article
- Two distinct modes of PERIOD recruitment onto dCLOCK reveal a novel role for TIMELESS in circadian transcription.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Sequential and compartment-specific phosphorylation controls the life cycle of the circadian CLOCK protein.The Journal of biological chemistry · 2009Article
- A role for the PERIOD:PERIOD homodimer in the Drosophila circadian clock.PLoS biology · 2009Article
- Remodeling the clock: coactivators and signal transduction in the circadian clockworks.Die Naturwissenschaften · 2009Review
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the Drosophila circadian clock, period (per) and its partner, timeless (tim), play a central role in the negative limb of an autoregulatory feedback loop. Unlike per, the dosage of which affects the frequency (tau) of the circadian cycle, we found that increasing copies of the tim gene has no effect on clock period length. The use of the tim promoter to express per results in a shortening of circadian period, also indicating that the regulation of tim is different from that of per. Drosophila TIM is similar to the mammalian circadian protein mPER2 in that it shuttles independently between the nucleus and cytoplasm both in vivo and in vitro. Contrary to the current model that PER and TIM heterodimerization is a prerequisite for their nuclear entry, PER is not required to transport TIM into nuclei, although it influences TIM localization and vice versa. Blocking nuclear export led to increased nuclear expression of TIM in S2 cells and in wild-type and per01 larvae, suggesting that PER may be required for nuclear retention of TIM. Unlike PER, nuclear TIM alone has no ability to repress transcription. We propose that TIM drives cycles of PER expression by regulating its stability, and in turn, PER retains TIM in the nucleus, either for the regulation of its own stability or for a novel nuclear role of TIM.
Indexed as
Identifiers
12944510PMC6740606W2113578944What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.