ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2003
Regulatory domains in the intergenic region of the oxytocin and vasopressin genes that control their hypothalamus-specific expression in vitro.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- FOXP1 is differentially active during development of murine vasopressin and oxytocin magnocellular neurons.iScience · 2026Article
- Characterization of a rat model expressing Cre recombinase in oxytocinergic neurons: NTac:SD-OxtNeuroscience · 2025Article
- Deciphering autism heterogeneity: a molecular stratification approach in four mouse models.Translational psychiatry · 2024Article
- Effects of bile duct ligation on the inhibitory control of supraoptic vasopressin neurons.Journal of neuroendocrinology · 2023Article
- Evaluating Methylation of the Oxytocin Receptor Gene and the Oxytocin Intergenic Region.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation.Scientific reports · 2021Article
- Cell-type specific expression of oxytocin and vasopressin genes: an experimental odyssey.Journal of neuroendocrinology · 2012Review
- Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.PloS one · 2012Article
- Article
- Dynamic DNA methylation programs persistent adverse effects of early-life stress.Nature neuroscience · 2009Article
- Effects of ciliary neurotrophic factor and leukemia inhibiting factor on oxytocin and vasopressin magnocellular neuron survival in rat and mouse hypothalamic organotypic cultures.Journal of neuroscience methods · 2009Article
- Characterization of the neurohypophysial hormone gene loci in elephant shark and the Japanese lamprey: origin of the vertebrate neurohypophysial hormone genes.BMC evolutionary biology · 2009Article
- Article
- Organotypic slice culture of the hypothalamic paraventricular nucleus of rat.Journal of veterinary science · 2007Article
- MicroRNAs and osmotic regulation.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Molecular morphology of neuronal apoptosis: analysis of caspase 3 activation during postnatal development of mouse cerebellar cortex.Journal of molecular histology · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies of oxytocin (OT) and vasopressin (VP) cell-specific gene expression in the hypothalamus using transgenic mouse and rat models focused attention on the intergenic region (IGR) as the site of critical enhancer elements. In this study, we used organotypic slice-explant cultures of rat hypothalamus as in vitro models, and particle-mediated gene transfer (biolistics) transfection methods to identify critical DNA sequences in the IGR between the OT and VP genes responsible for hypothalamic-specific gene expression. Reducing the 5' flanking region in the mouse VP gene from 3.5 kbp to 288 bp did not alter the efficacy of its expression in hypothalamic slices. All subsequent VP constructs were based on this 288 bp VP gene construct with changes made only to the IGR. These studies, which used various constructs with OT and VP promoters driving enhanced green fluorescent protein reporter gene expression, demonstrated that the IGR is necessary for OT and VP gene expression in hypothalamic slices in vitro. The DNA sequences in the IGR responsible for both OT and VP gene expression were located in a 178 bp domain immediately downstream of exon 3 of the VP gene. In addition, another domain in the IGR, 430 bp immediately downstream of exon 3 of the OT gene, contained a positive regulatory element for OT gene expression in the hypothalamus. Alignment of the DNA sequences in the 178 and 430 bp domains reveals four common sequences (motifs) that may be candidates for the putative enhancers in the IGR that regulate OT and VP gene hypothalamic-specific expression.
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12944509PMC6740589W1676344426What OpenQuestion holds
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.