ArticleMolecular and cellular biology2003
Phosphorelay-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Stress contingent changes in Hog1 pathway architecture and regulation in Candida albicans.PLoS pathogens · 2024Article
- Involvement of a Response Regulator VdSsk1 in Stress Response, Melanin Biosynthesis and Full Virulence inFrontiers in microbiology · 2019Article
- Deubiquitinase Ubp5 Is Required for the Growth and Pathogenicity of Cryptococcus gattii.PloS one · 2016Article
- An integrated view on a eukaryotic osmoregulation system.Current genetics · 2015Review
- Delayed Turnover of Unphosphorylated Ssk1 during Carbon Stress Activates the Yeast Hog1 Map Kinase Pathway.PloS one · 2015Article
- PLEIAD/SIMC1/C5orf25, a novel autolysis regulator for a skeletal-muscle-specific calpain, CAPN3, scaffolds a CAPN3 substrate, CTBP1.Journal of molecular biology · 2013Article
- An integrated pathway system modeling of Saccharomyces cerevisiae HOG pathway: a Petri net based approach.Molecular biology reports · 2013Article
- Response to hyperosmotic stress.Genetics · 2012Review
- A systems biology analysis of long and short-term memories of osmotic stress adaptation in fungi.BMC research notes · 2012Article
- Two-component elements mediate interactions between cytokinin and salicylic acid in plant immunity.PLoS genetics · 2012Article
- Pleiotropic effects of deubiquitinating enzyme Ubp5 on growth and pathogenesis of Cryptococcus neoformans.PloS one · 2012Article
- Remodeling of global transcription patterns of Cryptococcus neoformans genes mediated by the stress-activated HOG signaling pathways.Eukaryotic cell · 2009Article
- The cytokinin type-B response regulator PtRR13 is a negative regulator of adventitious root development in Populus.Plant physiology · 2009Article
- Master and commander in fungal pathogens: the two-component system and the HOG signaling pathway.Eukaryotic cell · 2008Review
- Abundance changes of the response regulator RcaC require specific aspartate and histidine residues and are necessary for normal light color responsiveness.Journal of bacteriology · 2008Article
- A bacterial control circuit integrates polar localization and proteolysis of key regulatory proteins with a phospho-signaling cascade.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Phosphorylated Ssk1 prevents unphosphorylated Ssk1 from activating the Ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway.Molecular and cellular biology · 2008Article
- Cytokinin regulates type-A Arabidopsis Response Regulator activity and protein stability via two-component phosphorelay.The Plant cell · 2007Article
- Function and regulation in MAPK signaling pathways: lessons learned from the yeast Saccharomyces cerevisiae.Biochimica et biophysica acta · 2007Review
- Regulation of cytotoxin expression by converging eukaryotic-type and two-component signalling mechanisms in Streptococcus agalactiae.Molecular microbiology · 2006Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Saccharomyces cerevisiae, a phosphorelay signal transduction pathway composed of Sln1p, Ypd1p, and Ssk1p, which are homologous to bacterial two-component signal transducers, is involved in the osmosensing mechanism. In response to high osmolarity, the phosphorelay system is inactivated and Ssk1p remains unphosphorylated. Unphosphorylated Ssk1p binds to and activates the Ssk2p mitogen-activated protein (MAP) kinase kinase kinase, which in turn activates the downstream components of the high-osmolarity glycerol response (HOG) MAP kinase cascade. Here, we report a novel inactivation mechanism for Ssk1p involving degradation by the ubiquitin-proteasome system. Degradation is regulated by the phosphotransfer from Ypd1p to Ssk1p, insofar as unphosphorylated Ssk1p is degraded more rapidly than phosphorylated Ssk1p. Ubc7p/Qri8p, an endoplasmic reticulum-associated ubiquitin-conjugating enzyme, is involved in the phosphorelay-regulated degradation of Ssk1p. In ubc7Delta cells in which the degradation is hampered, the dephosphorylation and/or inactivation process of the Hog1p MAP kinase is delayed compared with wild-type cells after the hyperosmotic treatment. Our results indicate that unphosphorylated Ssk1p is selectively degraded by the Ubc7p-dependent ubiquitin-proteasome system and that this mechanism downregulates the HOG pathway after the completion of the osmotic adaptation.
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