ArticleBiophysical journal2003
Assessment of multidrug resistance reversal using dielectrophoresis and flow cytometry.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 109 citations in OpenAlex.
- Article
- Glioma chemotherapeutic resistance is tied to membrane electrophysiological properties and glycosylation.Bioengineering & translational medicine · 2026Article
- Dielectrophoresis and ζ-potential as potential low-cost complements to patch clamp for measurement of cell electrophysiology.PloS one · 2026Article
- Meeting Review: "National Cancer Institute Conference on Cancer Bioelectricity" September 12, 2024.Bioelectricity · 2025Review
- Light-Emitting Diode Array with Optical Linear Detector Enables High-Throughput Differential Single-Cell Dielectrophoretic Analysis.Sensors (Basel, Switzerland) · 2024Article
- Visual and Quantitative Analysis of the Trapping Volume in Dielectrophoresis of Nanoparticles.Nano letters · 2024Article
- Label-free, non-contact determination of resting membrane potential using dielectrophoresis.Scientific reports · 2024Article
- Label-Free Enrichment of Circulating Tumor Plasma Cells: Future Potential Applications of Dielectrophoresis in Multiple Myeloma.International journal of molecular sciences · 2022Review
- Dielectrophoresis-Based SERS Sensors for the Detection of Cancer Cells in Microfluidic Chips.Biosensors · 2022Article
- A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting.Scientific reports · 2021Article
- Combined negative dielectrophoresis with a flexible SERS platform as a novel strategy for rapid detection and identification of bacteria.Analytical and bioanalytical chemistry · 2021Article
- Improvement of Background Solution for Optically Induced Dielectrophoresis-Based Cell Manipulation in a Microfluidic System.Frontiers in bioengineering and biotechnology · 2021Article
- A Prominent Cell Manipulation Technique in BioMEMS: Dielectrophoresis.Micromachines · 2020Review
- Differential Biophysical Behaviors of Closely Related Strains of Salmonella.Frontiers in microbiology · 2020Article
- New insights into anhydrobiosis using cellular dielectrophoresis-based characterization.Biomicrofluidics · 2019Article
- Isolation and identification of Listeria monocytogenes utilizing DC insulator-based dielectrophoresis.Analytica chimica acta · 2019Article
- Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device.Sensors (Basel, Switzerland) · 2018Article
- Cytometrical analysis of the adverse effects of indican, indoxyl, indigo, and indirubin on rat thymic lymphocytes.Toxicology research · 2018Article
- Breast cancer cell obatoclax response characterization using passivated-electrode insulator-based dielectrophoresis.Electrophoresis · 2017Article
- Single-Cell Electrical Phenotyping Enabling the Classification of Mouse Tumor Samples.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In cancer, multidrug resistance (MDR) is the simultaneous resistance of tumor cells to different natural product anticancer drugs that have no common structure. This is an impediment to the successful treatment of many human cancers. A common correlate of MDR is the overexpression of a membrane protein, P-glycoprotein. Many studies have shown that MDR can be reversed after the use of substrate analogs, called MDR modulators. However, our understanding of MDR modulation is incomplete. In this article, we examine the electrical properties of the human leukemic cells (K562) and its MDR counterpart (K562AR) using dielectrophoresis and flow cytometry (with a membrane potential sensitive dye, DIOC5), both before and after treatment with XR9576 (a P-glycoprotein-specific MDR-reversal agent). The results show significant differences in the cytoplasmic conductivity between the cell lines themselves, but indicate no significant changes after modulation therapy. We conclude that the process of MDR modulation is not associated with changes in the electrical properties of cancer cells. Moreover, the results demonstrate that using the flow cytometry method alone, with MDR cells, may produce artifactual results--whereas in combination with dielectrophoresis, the results show the role of MDR modulators in preventing drug efflux in MDR cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.