ArticleBiophysical journal2003
FTIR spectroscopy demonstrates biochemical differences in mammalian cell cultures at different growth stages.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- FTIR-ATR monitoring of growth-phase-dependent spectral responses ofRSC advances · 2026Article
- Metal Oxide Nanocomposites as Next-Generation Antimicrobial Agents Against Oral Cariogenic Pathogens: Mechanistic Actions of Ag-ZnO and Cu-ZnO onMaterials (Basel, Switzerland) · 2026Article
- Morphological and Chemical Changes in the Trophozoites and Cysts of Acanthamoeba Castellanii Induced by Camellia Sinensis Extracts.Acta parasitologica · 2025Article
- Cell Phase Identification in a Three-Dimensional Engineered Tumor Model by Infrared Spectroscopic Imaging.Analytical chemistry · 2023Article
- The Convergence of FTIR and EVs: Emergence Strategy for Non-Invasive Cancer Markers Discovery.Diagnostics (Basel, Switzerland) · 2022Review
- Metabolic sensor O-GlcNAcylation regulates erythroid differentiation and globin production via BCL11A.Stem cell research & therapy · 2022Article
- Studies on the Neuromodulatory Effects of Ginkgo biloba on Alterations in Lipid Composition and Membrane Integrity of Rat Brain Following Aluminium Neurotoxicity.Neurochemical research · 2020Article
- Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro.Toxics · 2020Article
- Alyssin and Iberin in Cruciferous Vegetables Exert Anticancer Activity in HepG2 by Increasing Intracellular Reactive Oxygen Species and Tubulin Depolymerization.Biomolecules & therapeutics · 2019Article
- Article
- 3D chemical imaging of the brain using quantitative IR spectro-microscopy.Chemical science · 2018Article
- Study of SH-SY5Y Cancer Cell Response to Treatment with Polyphenol Extracts Using FT-IR Spectroscopy.Biosensors · 2017Article
- Tracing overlapping biological signals in mid-infrared using colonic tissues as a model system.World journal of gastroenterology · 2017Article
- Quantifying Biochemical Alterations in Brown and Subcutaneous White Adipose Tissues of Mice Using Fourier Transform Infrared Widefield Imaging.Frontiers in endocrinology · 2017Article
- Article
- Application of mid-infrared (MIR) microscopy imaging for discrimination between follicular hyperplasia and follicular lymphoma in transgenic mice.The Analyst · 2015Article
- A biospectroscopic analysis of human prostate tissue obtained from different time periods points to a trans-generational alteration in spectral phenotype.Scientific reports · 2015Article
- Regulation of mammalian nucleotide metabolism and biosynthesis.Nucleic acids research · 2015Review
- Melatonin induces apoptosis through biomolecular changes, in SK-LU-1 human lung adenocarcinoma cells.Cell proliferation · 2014Article
- A FTIR imaging characterization of fibroblasts stimulated by various breast cancer cell lines.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We have observed differences in the infrared spectra of viable fibroblast cells depending on whether the cells were in the exponential (proliferating) or plateau (nonproliferating) phase of growth. The spectral changes were observed even after correcting for cell number and volume, ruling out these trivial explanations. Several of the changes occurred for both transformed and normal cell lines and were greater for the normal cell line. The biochemical basis of the spectral changes was estimated by fitting the cell spectra to a linear superposition of spectra for the major biochemical components of mammalian cells (DNA, RNA, protein, lipids, and glycogen). The ratios of RNA/lipid and protein/lipid increased when the cells were in the exponential phase compared to the plateau phase of growth. The fits of cell spectra to individual biochemical components also demonstrated that DNA is a relatively minor spectroscopic component as would be expected biochemically. Contrary to other reports in the literature, our data demonstrate that determining DNA content or structure using Fourier transform infrared spectroscopy data is difficult due to the relatively small amount of DNA and the overlap of DNA bands with the absorption bands of other biochemical components.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.