ArticleBiophysical journal2003
Kinetically differentiating influenza hemagglutinin fusion and hemifusion machines.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Non-uniform impact of extracellular osmotic variations at subcellular level.Cell death discovery · 2025Article
- Pleomorphism in Biological Units of Life: Morphological Heterogeneity in Cells Does Not Translate Uniformly to Subcellular Components.ACS omega · 2024Article
- Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling.The Journal of membrane biology · 2022Article
- Fusion dynamics of cubosome nanocarriers with model cell membranes.Nature communications · 2019Article
- Osmotic tolerance of avian erythrocytes to complete hemolysis in solute free water.Scientific reports · 2019Article
- Visualization and Sequencing of Membrane Remodeling Leading to Influenza Virus Fusion.Journal of virology · 2016Article
- Small-molecule photostabilizing agents are modifiers of lipid bilayer properties.Biophysical journal · 2013Article
- Review
- Intracellular curvature-generating proteins in cell-to-cell fusion.The Biochemical journal · 2011Article
- Imaging multiple intermediates of single-virus membrane fusion mediated by distinct fusion proteins.Microscopy research and technique · 2010Article
- Single-particle kinetics of influenza virus membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Mechanics of membrane fusion.Nature structural & molecular biology · 2008Review
- An allosteric rheostat in HIV-1 gp120 reduces CCR5 stoichiometry required for membrane fusion and overcomes diverse entry limitations.Journal of molecular biology · 2007Article
- Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegans.Molecular biology of the cell · 2007Article
- Heterogeneity of early intermediates in cell-liposome fusion mediated by influenza hemagglutinin.Biophysical journal · 2006Article
- Liposome reconstitution of a minimal protein-mediated membrane fusion machine.The EMBO journal · 2005Article
- Class II fusion protein of alphaviruses drives membrane fusion through the same pathway as class I proteins.The Journal of cell biology · 2005Article
- From genome to antivirals: SARS as a test tube.Drug discovery today · 2005Review
- Evidence that rabies virus forms different kinds of fusion machines with different pH thresholds for fusion.Journal of virology · 2004Article
- The energetics of membrane fusion from binding, through hemifusion, pore formation, and pore enlargement.The Journal of membrane biology · 2004Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Membrane fusion mediated by influenza virus hemagglutinin (HA) yields different phenotypes depending on the surface density of activated HAs. A key question is whether different phenotypes arise from different fusion machines or whether different numbers of identical fusion machines yield different probabilistic outcomes. If fusion were simply a less probable event than hemifusion, requiring a larger number of identical fusion machines to occur first, then two predictions can be made. First, fusion should have a shorter average delay time than hemifusion, since there are more machines. Second, fusion should have a longer execution time of lipid mixing after it begins than hemifusion, since the full event cannot be faster than the partial event. Using a new automated video microscopy technique, we simultaneously monitored many HA-expressing cells fusing with erythrocytes and identified individual cell pairs with either full or only partial redistribution of fluorescent lipids. The full lipid mixing phenotype also showed contents mixing, i.e., fusion. Kinetic screening of the digitized fluorescence data showed that the execution of lipid mixing after the onset is faster for fusion than hemifusion. We found no correlation between the delay times before the onset of lipid mixing and the final fusion phenotype. We also found that the execution time for fusion was faster than that for hemifusion. Thus, we provide the first experimental evidence for fusion and hemifusion arising from different machines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.