ArticleBiophysical journal2003
Ionic currents in the human serotonin transporter reveal inconsistencies in the alternating access hypothesis.
Article in Biophysical journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Electrophysiological Methods to Explore the Function of Monoamine Transporters.Advances in neurobiology · 2025Review
- Substrate Binding and Conformational Dynamics of the Monoamine Transporters.Advances in neurobiology · 2025Review
- Structural and functional perspectives on interactions between synthetic cathinones and monoamine transporters.Advances in pharmacology (San Diego, Calif.) · 2024Article
- Serotonin transport in the 21st century.The Journal of general physiology · 2019Review
- Kinetic Models of Secondary Active Transporters.International journal of molecular sciences · 2019Article
- Using CaPsychopharmacology · 2019Review
- A kinetic account for amphetamine-induced monoamine release.The Journal of general physiology · 2018Article
- Electrophysiological Actions of Synthetic Cathinones on Monoamine Transporters.Current topics in behavioral neurosciences · 2017Article
- Chloride requirement for monoamine transporters.Pflugers Archiv : European journal of physiology · 2016Review
- A current view of serotonin transporters.F1000Research · 2016Review
- The conservative view: is it necessary to implant a stent into the dopamine transporter?British journal of pharmacology · 2015Article
- 'Second-generation' mephedrone analogs, 4-MEC and 4-MePPP, differentially affect monoamine transporter function.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2015Article
- GLT-1 Transport Stoichiometry Is Constant at Low and High Glutamate Concentrations when Chloride Is Substituted by Gluconate.PloS one · 2015Article
- Unveiling the secret lives of glutamate transporters: VGLUTs engage in multiple transport modes.Neuron · 2014Article
- Evidence for a role of transporter-mediated currents in the depletion of brain serotonin induced by serotonin transporter substrates.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Article
- A quantitative model of amphetamine action on the 5-HT transporter.British journal of pharmacology · 2014Article
- 4-(4-(dimethylamino)phenyl)-1-methylpyridinium (APP+) is a fluorescent substrate for the human serotonin transporter.The Journal of biological chemistry · 2012Article
- Unifying concept of serotonin transporter-associated currents.The Journal of biological chemistry · 2012Article
- A conserved asparagine residue in transmembrane segment 1 (TM1) of serotonin transporter dictates chloride-coupled neurotransmitter transport.The Journal of biological chemistry · 2011Article
- Transmembrane domain 6 of the human serotonin transporter contributes to an aqueously accessible binding pocket for serotonin and the psychostimulant 3,4-methylene dioxymethamphetamine.The Journal of biological chemistry · 2010Article
Corrections and comments
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2 authors.
Funding
Abstract
We have investigated the conduction states of human serotonin transporter (hSERT) using the voltage clamp, cut-open frog oocyte method under different internal and external ionic conditions. Our data indicate discrepancies in the alternating access model of cotransport, which cannot consistently explain substrate transport and electrophysiological data. We are able simultaneously to isolate distinct external and internal binding sites for substrate, which exert different effects upon currents conducted by hSERT, in contradiction to the alternating access model. External binding sites of coupled Na ions are likewise simultaneously accessible from the internal and external face. Although Na and Cl are putatively cotransported, they have opposite effects on the internal face of the transporter. Finally, the internal K ion does not compete with internal 5-hydroxytryptamine for empty transporters. These data can be explained more readily in the language of ion channels, rather than carrier models distinguished by alternating access mechanisms: in a channel model of coupled transport, the currents represent different states of the same permeation path through hSERT and coupling occurs in a common pore.
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