ArticleJournal of the Association for Research in Otolaryngology : JARO2003
Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio).
Article in Journal of the Association for Research in Otolaryngology : JARO, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 251 papers.
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Who cites it
251 citing papers in PubMed, 476 citations in OpenAlex.
- Electrophysiological Profiling of Exocytosis during Early-Stage Development of the Zebrafish Lateral Line.eNeuro · 2026Article
- Transcriptomic profiling of inner ear hair cell spontaneous regeneration reveals the key role of dlx5a.Nature communications · 2026Article
- Reduced predator avoidance follows ocular and lateral line pathology in minnow species after diesel exposure.Scientific reports · 2026Article
- Discovery of a Novel Non-MET-Mediated Otoprotective Compound Against Aminoglycoside-Induced Ototoxicity.bioRxiv : the preprint server for biology · 2026Article
- Cell death modalities modulate inflammatory and regenerative programs in zebrafish sensory organs.Nature communications · 2026Article
- A sensorimotor instability drives a locomotor transition during fish development.Science advances · 2026Article
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- Neural Cell Adhesion Molecule Ncam1b Promotes Effective Hair Cell Regeneration in Zebrafish Neuromasts.International journal of molecular sciences · 2026Article
- The Lateral Line Facilitates Rapid Responses in Accelerating Fish Schools.Integrative and comparative biology · 2026Article
- Zebrafish neuromast development: a target for endocrine disrupting chemicals?Frontiers in toxicology · 2026Article
- Station Holding During Rheotaxis: A Sensitive Assay of Lateral Line Function in Larval Zebrafish.Bio-protocol · 2025Article
- Cohesin protein Smc3 influences kinocilial structure and function.Biology open · 2025Article
- Design and Synthesis of New Quinoxaline Analogs to Regenerate Sensory Auditory Hair Cells.Cells · 2025Article
- Electrophysiological profiling of exocytosis during early-stage development of the zebrafish lateral line.bioRxiv : the preprint server for biology · 2025Article
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- Mutation of the GDP-Fucose Biosynthesis GeneInternational journal of molecular sciences · 2025Article
- Research Progress on MET, Tip Link, and Stereocilia Complex with Special Reference to Zebrafish.International journal of molecular sciences · 2025Review
- Stem and progenitor cell proliferation are independently regulated by cell type-specific cyclinD genes.Nature communications · 2025Article
- The snakehead retrovirus promoter functions independently of the 3'ORF protein and its products are maternally inherited in transgenic zebrafish.PLoS pathogens · 2025Article
191 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mechanoreceptive hair cells are extremely sensitive to aminoglycoside antibiotics, including neomycin. Hair cell survival was assessed in larval wild-type zebrafish lateral line neuromasts 4 h after initial exposure to a range of neomycin concentrations for 1 h. Each of the lateral line neuromasts was scored in live fish for the presence or absence of hair cells using the fluorescent vital dye DASPEI to selectively label hair cells. All neuromasts were devoid of DASPEI-labeled hair cells 4 h after 500 microM neomycin exposure. Vital DASPEI staining was proportional to the number of hair cells per neuromast identified in fixed larvae using immunocytochemistry for acetylated tubulin and phalloidin labeling. The time course of hair cell regeneration in the lateral line neuromasts was also analyzed following neomycin-induced damage. Regenerated hair cells were first observed using live DASPEI staining 12 and 24 h following neomycin treatment. The potential role of proliferation in regenerating hair cells was analyzed. A 1 h pulse-fix protocol using bromodeoxyuridine (BrdU) incorporation was used to identify S-phase cells in neuromasts. BrdU incorporation in neomycin-damaged neuromasts did not differ from control neuromasts 4 h after drug exposure but was dramatically upregulated after 12 h. The proliferative cells identified during a 1 h period at 12 h after neomycin treatment were able to give rise to new hair cells by 24-48 h after drug treatment. The results presented here provide a standardized preparation for studying and identifying genes that influence vertebrate hair cell death, survival, and regeneration following ototoxic insults.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.