ArticleVirchows Archiv : an international journal of pathology2003
Gene expression profiles of microdissected pancreatic ductal adenocarcinoma.
Article in Virchows Archiv : an international journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers, 2 of them syntheses that pooled it.
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Who cites it
38 citing papers in PubMed, 2 syntheses or guidelines pooled it, 108 citations in OpenAlex.
- Meta-analysis of microarray data on pancreatic cancer defines a set of commonly dysregulated genes.Oncogene · 2005Pooled it
- Frequency of K-ras mutations in pancreatic intraductal neoplasias associated with pancreatic ductal adenocarcinoma and chronic pancreatitis: a meta-analysis.Neoplasia (New York, N.Y.) · 2005Pooled it
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- A Transcriptomics-Based Meta-Analysis Combined With Machine Learning Identifies a Secretory Biomarker Panel for Diagnosis of Pancreatic Adenocarcinoma.Frontiers in genetics · 2020Article
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- Four Decades After the Discovery of Regenerating Islet-Derived (Reg) Proteins: Current Understanding and Challenges.Frontiers in cell and developmental biology · 2019Review
- The 2016 John J. Abel Award Lecture: Targeting the Mechanical Microenvironment in Cancer.Molecular pharmacology · 2016Review
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- Tissue slide-based microRNA characterization of tumors: how detailed could diagnosis become for cancer medicine?Expert review of molecular diagnostics · 2014Review
- Evidence for the importance of personalized molecular profiling in pancreatic cancer.Pancreas · 2014Article
- Prognostic microRNAs in cancer tissue from patients operated for pancreatic cancer--five microRNAs in a prognostic index.World journal of surgery · 2012Article
- The rationale for targeting the LOX family in cancer.Nature reviews. Cancer · 2012Review
- Differentiation of multiple types of pancreatico-biliary tumors by molecular analysis of clinical specimens.Journal of molecular medicine (Berlin, Germany) · 2012Article
- Tissue and serum microRNAs in the Kras(G12D) transgenic animal model and in patients with pancreatic cancer.PloS one · 2011Article
- Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.Annals of surgical oncology · 2011Article
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- Functional analysis of LOXL2 in pancreatic carcinoma.International journal of colorectal disease · 2010Article
- Differential roles of cyclin D1 and D3 in pancreatic ductal adenocarcinoma.Molecular cancer · 2010Article
Corrections and comments
- Erratum issuedJesnowski, Ralf [corrected to Jesenofsky, Ralf]
Authors and funding
17 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In a search for new molecular markers of pancreatic ductal adenocarcinoma (PDAC), we compared the gene expression profiles of seven pancreatic carcinomas and one carcinoma of the papilla Vateri with those of duct cells from three non-neoplastic pancreatic tissues. In addition, the human pancreatic duct cell line and five PDAC cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2, HPAF) were examined. RNA was extracted from microdissected tissue or cultured cell lines and analysed using a custom-made Affymetrix Chip containing 3023 genes, of which 1000 were known to be tumour associated. Hierarchical clustering revealed 81 differentially expressed genes. Of all the genes, 26 were downregulated in PDAC and 14 were upregulated in PDAC. In PDAC cell lines versus normal pancreatic duct cells, 21 genes were downregulated and 20 were upregulated. Of these 81 differentially expressed genes, 15 represented human genes previously implicated in the tumourigenesis of PDAC. From the genes that were so far not known to be associated with PDAC tumorigenesis, we selected ADAM9 for further validation because of its distinct overexpression in tumour tissue. Using immunohistochemistry, the over-expressed gene, ADAM9, was present in 70% of the PDACs analysed. In conclusion, using microarray technology we were able to identify a set of genes whose aberrant expression was associated with PDAC and may be used to target the disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.