ArticleThe EMBO journal2003
Import of small Tim proteins into the mitochondrial intermembrane space.
Article in The EMBO journal, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 129 citations in OpenAlex.
- Galactokinase 1 Positively Regulates Mitochondrial Respiration by Phosphorylating TIMM13 as a Protein Kinase.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Targeting prohibitins activates the ISR through DELE1-HRI by impairing protein import into the mitochondrial matrix.Cell death and differentiation · 2026Article
- Privileged proteins with a second residence: dual targeting and conditional re-routing of mitochondrial proteins.The FEBS journal · 2024Review
- Oxidative protein folding in the intermembrane space of human mitochondria.FEBS open bio · 2024Review
- Identification of Timm13 protein translocase of the mitochondrial inner membrane as a potential mediator of liver fibrosis based on bioinformatics and experimental verification.Journal of translational medicine · 2023Article
- Mitochondrial protein translocation machinery: From TOM structural biogenesis to functional regulation.The Journal of biological chemistry · 2022Review
- Widespread use of unconventional targeting signals in mitochondrial ribosome proteins.The EMBO journal · 2022Article
- Article
- The chaperone-binding activity of the mitochondrial surface receptor Tom70 protects the cytosol against mitoprotein-induced stress.Cell reports · 2021Article
- Mitochondrial Quality Control in Neurodegenerative Diseases: Focus on Parkinson's Disease and Huntington's Disease.Frontiers in neuroscience · 2018Review
- Protein Translocation into the Intermembrane Space and Matrix of Mitochondria: Mechanisms and Driving Forces.Frontiers in molecular biosciences · 2017Review
- A disulfide bond in the TIM23 complex is crucial for voltage gating and mitochondrial protein import.The Journal of cell biology · 2016Article
- Article
- Mitochondrial Tim9 protects Tim10 from degradation by the protease Yme1.Bioscience reports · 2015Article
- A discrete pathway for the transfer of intermembrane space proteins across the outer membrane of mitochondria.Molecular biology of the cell · 2014Article
- Early targets of miR-34a in neuroblastoma.Molecular & cellular proteomics : MCP · 2014Article
- Common players in mitochondria biogenesis and neuronal protection against stress-induced apoptosis.Neurochemical research · 2014Review
- Folding and biogenesis of mitochondrial small Tim proteins.International journal of molecular sciences · 2013Review
- Protein import and oxidative folding in the mitochondrial intermembrane space of intact mammalian cells.Molecular biology of the cell · 2013Article
- Defective mitochondrial disulfide relay system, altered mitochondrial morphology and function in Huntington's disease.Human molecular genetics · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins of the intermembrane space (IMS) of mitochondria are typically synthesized without presequences. Little is known about their topogenesis. We used Tim13, a member of the 'small Tim protein' family, as model protein to investigate the mechanism of translocation into the IMS. Tim13 contains four conserved cysteine residues that bind a zinc ion as cofactor. Import of Tim13 did not depend on the membrane potential or ATP hydrolysis. Upon import into mitochondria Tim13 adopted a stably folded conformation in the IMS. Mutagenesis of the cysteine residues or pretreatment with metal chelators interfered with folding of Tim13 in vitro and impaired its import into mitochondria. Upon depletion of metal ions or modification of cysteine residues, imported Tim13 diffused back out of the IMS. We propose an import pathway in which (1) Tim13 can pass through the TOM complex into and out of the IMS in an unfolded conformation, and (2) cofactor acquisition stabilizes folding on the trans side of the outer membrane and traps Tim13 in the IMS, and drives unidirectional movement of the protein across the outer membrane of mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.