ArticleThe Journal of general physiology2003
Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2+.
Article in The Journal of general physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 126 citations in OpenAlex.
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- α-Synuclein kinetically regulates the nascent fusion pore dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- TMEM16F and dynamins control expansive plasma membrane reservoirs.Nature communications · 2021Article
- Presenilin 1 increases association with synaptotagmin 1 during normal aging.Neurobiology of aging · 2020Article
- Unraveling the mechanisms of calcium-dependent secretion.The Journal of general physiology · 2019Review
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- Presynaptic active zones of mammalian neuromuscular junctions: Nanoarchitecture and selective impairments in aging.Neuroscience research · 2018Review
- Synaptotagmin CaFrontiers in molecular neuroscience · 2018Review
- Doc2B acts as a calcium sensor for vesicle priming requiring synaptotagmin-1, Munc13-2 and SNAREs.eLife · 2017Article
- Tomosyn associates with secretory vesicles in neurons through its N- and C-terminal domains.PloS one · 2017Article
- Extension of Helix 12 in Munc18-1 Induces Vesicle Priming.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis.eLife · 2015Article
- A Post-Docking Role of Synaptotagmin 1-C2B Domain Bottom Residues R398/399 in Mouse Chromaffin Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Article
- Positively charged amino acids at the SNAP-25 C terminus determine fusion rates, fusion pore properties, and energetics of tight SNARE complex zippering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- PC12 cells that lack synaptotagmin I exhibit loss of a subpool of small dense core vesicles.Biophysical journal · 2014Article
- The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2+ sensor for fast neurosecretion by analyzing the intracellular Ca2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a mutated synaptotagmin C2A domain. The mutation (R233Q) causes a twofold increase in the KD of Ca2+-dependent phospholipid binding to the double C2A-C2B domain of synaptotagmin. Using photolysis of caged calcium and capacitance measurements we found that secretion from mutant cells had lower secretory rates, longer secretory delays, and a higher intracellular Ca2+-threshold for secretion due to a twofold increase in the apparent KD of the Ca2+ sensor for fast exocytosis. Single amperometric fusion events were unchanged. We conclude that Ca2+-dependent phospholipid binding to synaptotagmin 1 mirrors the intracellular Ca2+ dependence of exocytosis.
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