ArticleThe Journal of physiology2003
Sugar transport by mammalian members of the SLC26 superfamily of anion-bicarbonate exchangers.
Article in The Journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- The spatial pattern of cochlear amplification.Neuron · 2012Article
- Mammalian prestin is a weak Cl⁻/HCO₃⁻ electrogenic antiporter.The Journal of physiology · 2012Article
- Membrane cholesterol strongly influences confined diffusion of prestin.Biophysical journal · 2012Article
- Interaction between CFTR and prestin (SLC26A5).Biochimica et biophysica acta · 2010Article
- Fructose-induced hypertension: essential role of chloride and fructose absorbing transporters PAT1 and Glut5.Kidney international · 2008Article
- Hypotonic swelling of salicylate-treated cochlear outer hair cells.Hearing research · 2007Article
- Macrophage invasion contributes to degeneration of stria vascularis in Pendred syndrome mouse model.BMC medicine · 2006Article
- Distribution of pendrin in the organ of Corti of mice observed by electron immunomicroscopy.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2006Article
- Electromechanical models of the outer hair cell composite membrane.The Journal of membrane biologyReview
- Tuning in to the amazing outer hair cell: membrane wizardry with a twist and shout.The Journal of membrane biologyReview
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2 authors.
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Abstract
The mammalian cochlea contains a population of outer hair cells (OHCs) whose electromotility depends on an assembly of 'motor' molecules in the basolateral membrane of the cell. Named 'prestin', the molecule is a member of the SLC26 anion transporter superfamily. We show both directly and indirectly that SLC26A5, rat prestin, takes up hexoses when expressed in several cell lines. Direct measurements of labelled fructose transport into COS-7 cells expressing prestin are reported here. Indirect measurements, using imaging techniques, show that transfected HEK-293 or CHO-Ki cells undergo reversible volume changes when exposed to isosmotic glucose-fructose exchange. The observations are consistent with the sugar transport. A similar transport was observed using a C-terminal green fluorescent protein (GFP)-tagged pendrin (SLC26A4) construct. Cells transfected with GFP alone did not respond to sugars. The data are consistent with fructose being transported by prestin with an apparent Km=24 nm. From the voltage-dependent capacitance of transfected cells, we estimate that 250,000 prestin molecules were present and hence that the single transport rate is not more than 3000 fructose molecules s(-1). Comparison of the transfected cell swelling rates induced by fructose and by osmotic steps indicates that water was co-transported with sugar. We suggest that the structure of SLC26 family members allows them to act as neutral substrate transporters and may explain observed properties of cochlear hair cells.
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