ArticlePflugers Archiv : European journal of physiology2003
Measurement of rapid changes in cell volume by forward light scattering.
Article in Pflugers Archiv : European journal of physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 30 citations in OpenAlex.
- A Note of Caution: Gramicidin Affects Signaling Pathways Independently of Its Effects on Plasma Membrane Conductance.BioMed research international · 2021Article
- Evaluation of Demodex mite viability using motility and scattered light intensity.Experimental & applied acarology · 2019Article
- CLN3 loss disturbs membrane microdomain properties and protein transport in brain endothelial cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Real-time monitoring of epithelial cell-cell and cell-substrate interactions by infrared surface plasmon spectroscopy.Biophysical journal · 2010Article
- Measurement of the thickness and volume of adherent cells using transmission-through-dye microscopy.Pflugers Archiv : European journal of physiology · 2010Article
- Measurement and modeling of diffusion kinetics of a lipophilic molecule across rabbit cornea.Pharmaceutical research · 2010Article
- Dynamic regulation of barrier integrity of the corneal endothelium.Optometry and vision science : official publication of the American Academy of Optometry · 2010Review
- Measurement of intrinsic optical backscattering characteristics of cells using fiber-guided near infrared light.Biomedical engineering online · 2010Article
- Lack of threshold for anisotonic cell volume regulation.The Journal of membrane biology · 2006Article
- Optophysiology: depth-resolved probing of retinal physiology with functional ultrahigh-resolution optical coherence tomography.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Light scattering is an empirical technique employed to measure rapid changes in cell volume. This study describes a new configuration for the method of light scattering and its corroboration by measurements of cell height (as a measure of cell volume). Corneal endothelial cells cultured on glass cover-slips were mounted in a perfusion chamber on the stage of an inverted microscope. A beam of light was focused on the cells from above the stage at an angle of 40 degrees to the plane of the stage. The scattered light intensity (SLI), captured by the objective and referred to as forward light scatter (FLS), increased and decreased in response to hyposmotic and hyperosmotic shocks, respectively. The rapid increase and decrease in SLI corresponded to cell swelling and shrinkage, respectively. Subsequently, SLI decreased and increased as expected for a regulatory volume decrease (RVD) and increase (RVI), respectively. These data are in agreement with measurements of cell height, demonstrating that the method of light scatter in FLS mode is useful for monitoring rapid changes in cell volume of cultured cells. Changes in SLI caused by gramicidin were consistent with cell volume changes induced by equilibration of NaCl and KCl concentrations across the cell membrane. Similarly, an additional decrease in SLI was recorded during RVD upon increasing K+ conductance by valinomycin. Decreasing K+ conductance of the cell membrane with Ba2+ changed the time course of SLI consistent with the effect of the K+ channel blocker on RVD. Bumetanide and dihydro-ouabain inhibited increases in SLI during RVI. In conclusion, FLS is a valid method for qualitative analysis of cell volume changes with a high time resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.