ArticleThe American journal of pathology2003
Ikaros isoforms in human pituitary tumors: distinct localization, histone acetylation, and activation of the 5' fibroblast growth factor receptor-4 promoter.
Article in The American journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 78 citations in OpenAlex.
- Insights intoInternational journal of molecular sciences · 2025Article
- Human growth hormone proteoform pattern changes in pituitary adenomas: Potential biomarkers for 3P medical approaches.The EPMA journal · 2021Article
- Genomics and Epigenomics of Pituitary Tumors: What Do Pathologists Need to Know?Endocrine pathology · 2021Review
- Quantitative Acetylomics Revealed Acetylation-Mediated Molecular Pathway Network Changes in Human Nonfunctional Pituitary Neuroendocrine Tumors.Frontiers in endocrinology · 2021Article
- Ikaros is heterogeneously expressed in lung adenocarcinoma and is involved in its progression.The Journal of international medical research · 2020Article
- An investigation of methylation pattern changes in the IKZF1 promoter in patients with childhood B-cell acute lymphoblastic leukemia.Blood research · 2019Article
- Retraction. Targeted expression of a human pituitary tumor-derived isoform of FGF receptor-4 recapitulates pituitary tumorigenesis.The Journal of clinical investigation · 2015Article
- Two functional loci in the promoter of EPAS1 gene involved in high-altitude adaptation of Tibetans.Scientific reports · 2014Article
- Inhibitory effects of transcription factor Ikaros on the expression of liver cancer stem cell marker CD133 in hepatocellular carcinoma.Oncotarget · 2014Article
- Genomic approaches to problems in pituitary neoplasia.Endocrine pathology · 2014Review
- FGFR4 polymorphic variants modulate phenotypic features of Cushing disease.Molecular endocrinology (Baltimore, Md.) · 2014Article
- The cancer-associated FGFR4-G388R polymorphism enhances pancreatic insulin secretion and modifies the risk of diabetes.Cell metabolism · 2013Article
- Genome-wide two-locus epistasis scans in prostate cancer using two European populations.Human genetics · 2012Article
- CtBP1 interacts with Ikaros and modulates pituitary tumor cell survival and response to hypoxia.Molecular endocrinology (Baltimore, Md.) · 2012Article
- Nuclear localization of folate receptor alpha: a new role as a transcription factor.Scientific reports · 2012Article
- The FGFR4-G388R polymorphism promotes mitochondrial STAT3 serine phosphorylation to facilitate pituitary growth hormone cell tumorigenesis.PLoS genetics · 2011Article
- Ikaros isoforms: The saga continues.World journal of biological chemistry · 2011Article
- Regulation of Ikaros function by casein kinase 2 and protein phosphatase 1.World journal of biological chemistry · 2011Article
- Anterior pituitary adenomas: inherited syndromes, novel genes and molecular pathways.Expert review of endocrinology & metabolism · 2010Article
- Histone-acetylated control of fibroblast growth factor receptor 2 intron 2 polymorphisms and isoform splicing in breast cancer.Molecular endocrinology (Baltimore, Md.) · 2009Article
Corrections and comments
- Expression of concern · 2015-07-26Duplication of Data · Duplication of/in Image · Investigation by Company/Institution · Investigation by Journal/Publisher · Objections by Third Party ·
- Commented on byNote of concern.2015
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted expression of a human pituitary tumor derived-fibroblast growth factor receptor-4 (FGFR4) recapitulates pituitary tumorigenesis. We have shown that FGFR4 is a target for Ikaros, a zinc finger-containing transcription factor that localizes to heterochromatin regions and participates in higher order chromatin complexes and control of gene expression. We report here the expression of Ikaros and functional differences between its alternatively spliced variants in human pituitary tumors. Ik1 expression was detected in human pituitary tumors and we also identified a truncated isoform consistent with the non-DNA-binding Ik6 isoform in a subset of adenomas by reverse transcriptase-polymerase chain reaction, sequencing, and Western immunoblotting. Transfection of Ik6 in GH4 pituitary cells resulted in predominantly cytoplasmic expression as compared to Ik1, which resulted in exclusively nuclear expression as determined by immunofluorescence and immunoblotting of fractionated protein. Immunohistochemistry of primary human pituitary adenomas localized Ikaros expression to the nuclear compartment but also in the cytoplasm, the latter consistent with Ik6. Expression of Ikaros and truncated non-DNA-binding isoforms was also suggested by electromobility shift assays using nuclear proteins from primary human pituitary adenomas. Ik6 resulted in reversal of the effects of Ik1 on wild-type 5' FGFR4 promoter activity, histone acetylation, and regulation of the endogenous gene. We conclude that dominant-negative Ik6 isoforms with their distinct localization and effects on Ik1 action may contribute to the altered expression of FGFR4 and possibly other target genes in human pituitary tumors.
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