ArticleThe American journal of pathology2003
Significance of immunological detection of human telomerase reverse transcriptase: re-evaluation of expression and localization of human telomerase reverse transcriptase.
Article in The American journal of pathology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 74 citations in OpenAlex.
- TERT Amplification a Risk Stratification Marker in Papillary Thyroid Carcinoma, Significantly Correlated with Tumor Recurrence and Survival.Endocrine pathology · 2025Article
- Review of hTERT-Immortalized Cells: How to Assess Immortality and Confirm Identity.International journal of molecular sciences · 2024Review
- Deletion of 184-188 Nucleotides of Human Telomerase RNA Does Not Affect the Telomerase Functioning.Doklady. Biochemistry and biophysics · 2023Article
- A Pan-Cancer Study of Somatic TERT Promoter Mutations and Amplification in 30,773 Tumors Profiled by Clinical Genomic Sequencing.The Journal of molecular diagnostics : JMD · 2021Article
- The Regulation of ROS- and BECN1-Mediated Autophagy by Human Telomerase Reverse Transcriptase in Glioblastoma.Oxidative medicine and cellular longevity · 2021Article
- Generation of an immortalized human choroid endothelial cell line (iChEC-1) using an endothelial cell specific promoter.Microvascular research · 2019Article
- Neural Stem Cells of the Subventricular Zone as the Origin of Human Glioblastoma Stem Cells. Therapeutic Implications.Frontiers in oncology · 2019Review
- In Vitro Assessment of the Expression and T Cell Immunogenicity of the Tumor-Associated Antigens BORIS, MUC1, hTERT, MAGE-A3 and Sp17 in Uterine Cancer.International journal of molecular sciences · 2016Article
- Nuclear translocation of telomerase reverse transcriptase is a critical process in lymphatic metastasis of nasopharyngeal carcinoma.Oncology letters · 2015Article
- Endometrial stem cells in regenerative medicine.Journal of biological engineering · 2014Review
- Novel telomerase-increasing compound in mouse brain delays the onset of amyotrophic lateral sclerosis.EMBO molecular medicine · 2012Article
- A functional tandem-repeats polymorphism in the downstream of TERT is associated with the risk of nasopharyngeal carcinoma in Chinese population.BMC medicine · 2011Article
- Characterization of human multicentric osteosarcoma using newly established cells derived from multicentric osteosarcoma.Journal of cancer research and clinical oncology · 2011Article
- Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice.Journal of assisted reproduction and genetics · 2010Article
- Telomerase downregulation in cancer brain stem cell.Molecular and cellular biochemistry · 2009Article
- Immunohistochemical expression of ubiquitin and telomerase in cervical cancer.Virchows Archiv : an international journal of pathology · 2009Article
- Progression of astrocytomas and meningiomas: an evaluation in vitro.Cell proliferation · 2007Article
- Novel functional single nucleotide polymorphisms in the latent transforming growth factor-beta binding protein-1L promoter: effect on latent transforming growth factor-beta binding protein-1L expression level and possible prognostic significance in ovarian cancer.The Journal of molecular diagnostics : JMD · 2006Article
- Potential of telomerase expression and activity in cervical specimens as a diagnostic tool.Journal of clinical pathology · 2005Article
- Expression of human telomerase catalytic protein in gallbladder carcinogenesis.Journal of clinical pathology · 2005Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human telomerase reverse transcriptase (hTERT) is a catalytic subunit of telomerase and is a potentially useful diagnostic marker for cancers. There have been few studies in which immunological detection of hTERT has been attempted and its subcellular localization has not been precisely defined. In the present study, we re-evaluated expression and localization of hTERT in cancer and normal cells using a newly developed antibody. Immunohistochemistry revealed that hTERT is expressed in approximately 80% of gynecological cancers, but some premalignant lesions exhibited weak expression of hTERT. Interestingly, not only nuclei but also cytoplasm of cancer cells were positive for hTERT staining. This finding was supported by the results of Western blot analysis of cell lines, in which both nuclear and cytoplasmic extracts exhibited significant hTERT bands. Cytoplasmic hTERT in cancer cells may be functional because the telomeric repeat amplification protocol assay of cytoplasmic extracts showed high levels of telomerase activity. Unexpectedly, not all normal primary cells and telomerase-negative cancer cell lines lacked hTERT expression; some exhibited weak TERT signals. In Western analysis, hTERT signals did not always correlate with telomerase activity of the various cell types. These findings suggest that functional hTERT is expressed in both the nucleus and cytoplasm of cancer cells and that hTERT expression does not strictly reflect telomerase activity. Further analysis is needed to clarify the biological significance of cytoplasmic hTERT.
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